'Psoriasis 1' reduces psoriasis‑like skin inflammation by inhibiting the VDR‑mediated nuclear NF‑κB and STAT signaling pathways

Molecular Medicine Reports
Wen SunXushan Cha

Abstract

'Psoriasis 1', a Chinese herbal medicine (CHM) formulation, is extensively used to treat psoriasis in China. Although this CHM formulation yields good therapeutic effect, the underlying mechanism of how this works remains unknown. The present study aimed to test the hypothesis that the CHM formulation 'psoriasis 1' inhibits vitamin D receptor (VDR)‑mediated inflammation in psoriasis. To test this, a model of psoriasis was established by stimulating keratinocytes (HaCaT cells) with tumor necrosis factor (TNF)‑α; these cells were subsequently transfected with a lentiviral VDR RNA interference expression vector. The expression levels of 25‑hydroxyvitamin D3 (25HVD3), TNF‑α, interleukin (IL)‑4, IL‑1, IL‑17C, IL‑23 and IL‑6 were measured using ELISA, and the expression levels of VDR, inhibitor of nuclear factor (NF)‑κB (IKK), NF‑κB, signal transducer and activator of transcription (STAT) 3 and STAT4 were measured using reverse transcription‑quantitative polymerase chain reaction analysis and western blotting. It was observed that 'psoriasis 1' downregulated the concentrations of TNF‑α, IFN‑γ, IL‑22, IL‑17C, IL‑1β and IL‑4, and upregulated the concentration of 25HVD3; furthermore, 'psoriasis 1' downregulated the expression levels of ...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Sep 3, 2003·Clinical and Experimental Dermatology·T W Tse
Mar 12, 2004·Cancer Letters·Florian R Greten, Michael Karin
Mar 2, 2006·Annales de dermatologie et de vénéréologie·A RozieresJ-F Nicolas
Oct 4, 2006·Pediatric Dermatology·Yin-Ku LinJong-Hwei Su Pang
Mar 24, 2007·The Journal of Clinical Investigation·Swarnali AcharyyaDenis C Guttridge
Jan 20, 2009·Recent Patents on Inflammation & Allergy Drug Discovery·Daisuke Tsuruta
Mar 27, 2009·The Journal of Investigative Dermatology·Antonella Di CesareFrank O Nestle
Jul 17, 2009·The American Journal of Chinese Medicine·Stevenson XutianWozniak Louise
Jul 31, 2009·The New England Journal of Medicine·Frank O NestleJonathan Barker
Oct 9, 2009·The Journal of Investigative Dermatology·James T ElderRajan P Nair
Nov 10, 2009·Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan·Guang-Zhong ZhangRuo-Yu Li
Dec 17, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Sif HansdottirGary W Hunninghake
Dec 19, 2009·Cytokine & Growth Factor Reviews·Sergei I Grivennikov, Michael Karin
Jan 21, 2010·British Journal of Cancer·Y Wu, B P Zhou
Jan 26, 2010·Actas dermo-sifiliográficas·R K H MakF O Nestle
Apr 3, 2012·Chinese Journal of Integrative Medicine·Chuan-Jian LuJing-Wen Deng
Apr 3, 2012·Chinese Journal of Integrative Medicine·Brian H MayCharlie C L Xue
Jan 22, 2013·The Journal of Steroid Biochemistry and Molecular Biology·Qi WangHaiyang Yu
Aug 29, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Jason Jingjie YuChuanjian Lu
Feb 13, 2014·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Erin T LandisSarah Taylor
Aug 14, 2015·Scientific Reports·Fatemeh FekrmandiJohn H White
Nov 9, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Shu-Wen WengHung-Rong Yen

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Citations

Feb 28, 2020·Experimental and Therapeutic Medicine·Wei PanXingyu Song
Jul 14, 2020·Evidence-based Complementary and Alternative Medicine : ECAM·Lin ZhouDisheng Tao

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Methods Mentioned

BETA
transfection
ELISA
Protein Assay

Software Mentioned

GraphPad Prism
SPSS

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