PMID: 28502301May 16, 2017Paper

Psychological distress promotes proliferation and invasion of glioma by upregulating lactate deoxygenase A

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Ping WangJunhong Dong

Abstract

Objective To investigate the role of lactate deoxygenase A (LDHA) expression in the proliferation and invasion of glioma promoted by the psychological distress. Methods The glioma-bearing nude mice were divided into tumor bearing group, stress tumor bearing group, negative control, short hairpin RNA (shRNA)-lactate dehydrogenase A (LDHA) group and shRNA-LDHA stress group. Four weeks after stress, the tumor size of each group was measured; meanwhile, norepinephrine (NE) andadrenaline (EPI) in the blood were detected by ELISA; lactic acid in the tumor tissue was determined by colorimetry. And the expression level of LDHA was detected by Western blotting. The proliferation of glioma LN229 cells stimulated by NE was detected by CCK-8 assay and plate clone formation assay; Transwell(TM) assay was used to test the invasive ability of LN229 cells. The level of LDHA mRNA was detected by real-time quantitative PCR. The expressions of LDHA, ERK1/2 and hypoxia-induced factor-1α (HIF-1α) were observed using Western blotting. By constructing the reporter gene, we studied the mechanism of LDHA expression regulated by NE. Results The tumor size and the levels of EPI, NE, lactic acid and LDHA in the stress group significantly increased as comp...Continue Reading

Related Concepts

Related Papers

Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Xiaoyuan DengFangzhou Song
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Ruirui ShiShuyang Zhu
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Zhibin TangZhiwei Chen
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology
Yayun JiangYan Zhang
© 2022 Meta ULC. All rights reserved