Psychrophilic Pseudomonas syringae requires trans-monounsaturated fatty acid for growth at higher temperature

Extremophiles : Life Under Extreme Conditions
Madanahally D KiranS Shivaji

Abstract

A psychrophilic bacterium, Pseudomonas syringae (Lz4W) from Antarctica, was used as a model system to establish a correlation, if any, between thermal adaptation, trans-fatty acid content and membrane fluidity. In addition, attempts were made to clone and sequence the cti gene of P. syringae (Lz4W) so as to establish its characteristics with respect to the cti of other Pseudomonas spp. and also to in vitro mutagenize the cti gene so as to generate a cti null mutant. The bacterium showed increased proportion of saturated and trans-monounsaturated fatty acids when grown at 28 degrees C compared to cells grown at 5 degrees C, and the membrane fluidity decreased with growth temperature. In the mutant, the trans-fatty acid was not synthesized, and the membrane fluidity also decreased with growth temperature, but the decrease was not to the extent that was observed in the wild-type cells. Thus, it would appear that synthesis of trans-fatty acid and modulation of membrane fluidity to levels comparable to the wild-type cells is essential for growth at higher temperatures since the mutant exhibits growth arrest at 28 degrees C. In fact, the cti null mutant-complemented strain of P. syringae (Lz4W-C30b) that was capable of synthesizing t...Continue Reading

References

Jun 1, 1978·Journal of Bacteriology·R A Makula
Jan 1, 1992·Applied Microbiology and Biotechnology·R DiefenbachH J Rehm
Jan 30, 1990·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·N J Russell
Mar 30, 1990·Gene·R DeFeyterD W Gabriel
Mar 1, 1989·Applied and Environmental Microbiology·S ShivajiP M Bhargava
Sep 10, 1974·Biochemistry·J M Vanderkooi, J B Callis
Feb 26, 1974·Biochimica Et Biophysica Acta·H J Galla, E Sackmann
Sep 30, 1982·Biochemical and Biophysical Research Communications·S VijayasarathyP Balaram
Jan 1, 1994·Critical Reviews in Microbiology·M Suutari, S Laakso
Feb 1, 1996·Lipids·H Keweloh, H J Heipieper
Feb 14, 1997·The Journal of Biological Chemistry·J L RamosA Fernández-Barrero
Nov 12, 1998·Trends in Biochemical Sciences·L VighJ L Harwood
Nov 26, 1998·International Journal of Systematic Bacteriology·I YumotoH Okuyama
Jun 15, 1999·Biochimica Et Biophysica Acta·J L van de VossenbergW N Konings
Aug 12, 2000·International Journal of Systematic and Evolutionary Microbiology·G S ReddyS Shivaji
Apr 3, 2001·Current Opinion in Microbiology·J L RamosA Segura
Nov 6, 2001·Applied Microbiology and Biotechnology·L Beney, P Gervais
Apr 6, 2002·Current Opinion in Microbiology·John E Cronan
Dec 3, 2002·Journal of Microbiological Methods·T J DenichJ T Trevors
Feb 20, 2003·Journal of Bacteriology·Angelika von WallbrunnHermann J Heipieper

❮ Previous
Next ❯

Citations

Jan 6, 2010·Archives of Microbiology·S Shivaji, Jogadhenu S S Prakash
Jun 17, 2010·Archives of Microbiology·V R SundareswaranS Shivaji
May 12, 2009·Applied and Environmental Microbiology·Ashish K SinghS Shivaji
Sep 23, 2011·Biotechnology for Biofuels·Mary J Dunlop
Dec 1, 2009·Research in Microbiology·Ashish Kumar Singh, Sisinthy Shivaji
Feb 2, 2006·International Journal of Systematic and Evolutionary Microbiology·Pratima GuptaSisinthy Shivaji
May 17, 2017·Extremophiles : Life Under Extreme Conditions·Melvin F SiliakusServé W M Kengen
Nov 11, 2020·Environmental Microbiology·Theetha L PavankumarJohn E Hallsworth
Jul 14, 2007·Progress in Biophysics and Molecular Biology·N C S MykytczukG D Ferroni

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.