PTAP motif duplication in the p6 Gag protein confers a replication advantage on HIV-1 subtype C

The Journal of Biological Chemistry
Shilpee SharmaUdaykumar Ranga

Abstract

HIV-1 subtype C (HIV-1C) may duplicate longer amino acid stretches in the p6 Gag protein, leading to the creation of an additional Pro-Thr/Ser-Ala-Pro (PTAP) motif necessary for viral packaging. However, the biological significance of a duplication of the PTAP motif for HIV-1 replication and pathogenesis has not been experimentally validated. In a longitudinal study of two different clinical cohorts of select HIV-1 seropositive, drug-naive individuals from India, we found that 8 of 50 of these individuals harbored a mixed infection of viral strains discordant for the PTAP duplication. Conventional and next-generation sequencing of six primary viral quasispecies at multiple time points disclosed that in a mixed infection, the viral strains containing the PTAP duplication dominated the infection. The dominance of the double-PTAP viral strains over a genetically similar single-PTAP viral clone was confirmed in viral proliferation and pairwise competition assays. Of note, in the proximity ligation assay, double-PTAP Gag proteins exhibited a significantly enhanced interaction with the host protein tumor susceptibility gene 101 (Tsg101). Moreover, Tsg101 overexpression resulted in a biphasic effect on HIV-1C proliferation, an enhance...Continue Reading

References

Apr 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·H G GöttlingerW A Haseltine
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Apr 20, 2000·AIDS Research and Human Retroviruses·M P De BaarJ Goudsmit
Jun 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·L VerPlankC A Carter
Jan 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Dimiter G DemirovEric O Freed
Mar 8, 2003·Journal of Clinical Microbiology·Oscar GallegoVincent Soriano
Apr 30, 2003·Protein Science : a Publication of the Protein Society·Morten NielsenOle Lund
Aug 6, 2003·The Journal of Cell Biology·Owen PornillosWesley I Sundquist
Sep 25, 2003·Cell·Bettina StrackHeinrich G Göttlinger
Nov 25, 2003·HIV Clinical Trials·Oscar GallegoVincent Soriano
Feb 14, 2004·Journal of Virology·Udaykumar RangaVinayaka R Prasad
Mar 24, 2004·Nature Reviews. Microbiology·Viviana Simon, David D Ho
Dec 9, 2004·AIDS Research and Human Retroviruses·Natalia MarloweUNKNOWN Adult AIDS Clinical Trials Groups
Mar 30, 2005·AIDS Research and Human Retroviruses·Robert C ColgroveSeth L Welles
Aug 17, 2005·AIDS·Nagadenahalli Byrareddy SiddappaUdaykumar Ranga
Sep 1, 2005·AIDS Research and Human Retroviruses·Tamara FlysSusan H Eshleman
Oct 18, 2005·AIDS Research and Human Retroviruses·Africa HolguínVincent Soriano
Jul 15, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jamil S SaadMichael F Summers
Apr 5, 2007·Nature Protocols·Laurie LamoreauxRichard Koup
May 21, 2008·Proceedings of the National Academy of Sciences of the United States of America·Brandon F KeeleGeorge M Shaw
Jan 16, 2009·Biochemical Society Transactions·Yoshiko UsamiHeinrich G Göttlinger
Jun 3, 2009·The Journal of Experimental Medicine·Jesus F Salazar-GonzalezGeorge M Shaw
Jun 17, 2009·Cell Host & Microbe·Paul D Bieniasz
Oct 29, 2009·Proceedings of the National Academy of Sciences of the United States of America·Nolwenn JouvenetPaul D Bieniasz

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