PMID: 1204623Nov 15, 1975Paper

Pt(CN)2-4 and Au(CN)-2: potential general probes for anion-binding sites of proteins. 35Cl and 81Br nuclear-magnetic-resonance studies

European Journal of Biochemistry
J E NorneM Zeppezauer

Abstract

Nuclear magnetic quadrupole relaxation appears to be a general method for studying the binding of anions to proteins. This is shown by the increase in transverse quadrupole relaxation rate of 35Cl- and 81Br- in the presence of horse liver alcohol dehydrogenase, lysozyme, trypsin, alpha-chymotrypsin, human carbonic anhydrase, fructose-1,6-bisphosphate aldolase and human serum albumin. Of the many possible binding sites at the surface of a protein (e.g. positively charged amino acid side-chains) only a few account for the main part of the relaxation enhancement. This is shown by the decrease in 35Cl- and 81Br- relaxation rate on addition of functional ligands. Large, kinetically inert, complex anions like Pt(CN)2-4 and Au(CN)-2 are found to act as strong competitors towards halogen ions for the high-affinity anion binding sites of a number of proteins. Titrations with complex anions following the 35Cl- or 81Br- relaxation rates are found to be helpful in attempts to elucidate binding mechanisms. Especially, the complex anions may be useful probes for the discrimination between general and metallic anion binding sites in proteins and they also permit correlation of information from X-ray investigations of crystals with that from p...Continue Reading

References

Jun 1, 1974·Archives of Biochemistry and Biophysics·J E NorneB Lindman
Sep 24, 1969·Biochemical and Biophysical Research Communications·M ZeppezauerI Lindqvist
Dec 17, 1971·Biochemical and Biophysical Research Communications·R L Ward, J A Happe
Aug 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·C I BrändénA Akeson
Nov 1, 1974·European Journal of Biochemistry·R EinarssonC I Brändén
Aug 25, 1974·FEBS Letters·H EklundC I Brändén
Feb 16, 1972·Nature: New Biology·C C BlakeR K Scopes
Oct 1, 1973·Biochemical and Biophysical Research Communications·J T Gerig, L Kwock
Jun 1, 1972·Archives of Biochemistry and Biophysics·R L Ward, M D Cull
Mar 17, 1973·Biochemical and Biophysical Research Communications·R L Ward, P L Whitney
Apr 16, 1974·European Journal of Biochemistry·P O GunnarssonM Zeppezauer
Dec 1, 1970·Archives of Biochemistry and Biophysics·G L Cottam, R L Ward
May 1, 1971·Analytical Biochemistry·J L Sudmeier, J J Pesek
Oct 1, 1971·Archives of Biochemistry and Biophysics·L O AnderssonS Johansson
Oct 15, 1971·Biochemical and Biophysical Research Communications·R G Bryant, S Rajender
May 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·T R Stengle, J D Baldeschwieler
Nov 6, 1969·Biochemical and Biophysical Research Communications·R G BryantT R Stengle
Jan 6, 1970·Biochemical and Biophysical Research Communications·G Gillberg
Feb 1, 1969·Canadian Journal of Biochemistry·W D EllisJ S Martin
Feb 12, 1969·Journal of the American Chemical Society·R G Bryant
Jun 1, 1969·Archives of Biochemistry and Biophysics·G L Cottam, R L Ward
Jun 7, 1967·Journal of the American Chemical Society·T R Stengle, J D Baldeschwieler

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Citations

Oct 1, 1976·European Journal of Biochemistry·J Kvassman, G Pettersson
Nov 30, 2018·Physical Chemistry Chemical Physics : PCCP·Tadeja JancNatalie Malikova
Jan 29, 1999·Physiological Reviews·D C DawsonM K Mansoura
Aug 1, 1980·Analytical Biochemistry·T RajR G Bryant
Sep 1, 1979·Archives of Biochemistry and Biophysics·J E NorneB Lindman

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