PTEN regulates apoptotic cell death through PI3-K/Akt/GSK3β signaling pathway in DMH induced early colon carcinogenesis in rat

Experimental and Molecular Pathology
Manpreet Kaur Saini, Sankar Nath Sanyal

Abstract

Phosphatidylinositol 3-kinase (PI3-K) and Akt (protein kinase B), are both essential signaling molecules that are up-regulated in various cancers. Here, we examined the molecular mechanisms by which PI3-K and Akt expression are regulated by glycogen synthase kinase-3β (GSK-3β) and the phosphatase and tensin homolog deleted on chromosome 10 (PTEN) in the early stages of experimental colon carcinogenesis. 1,2-dimethylhydrazine (DMH) was utilized for the induction of colon cancer while piroxicam, a traditional non-steroidal anti-inflammatory drug and c-phycocyanin, a biliprotein from Spirulina platensis (cyanobacterium) as the chemopreventive agents. Western blotting and immunofluorescence results indicated that the expression of PI3-K and Akt was promoted in the DMH group while least apoptosis was detected in this group as analyzed by Hoechst 33342-propidium iodide co-staining. DMH group further detected lower GSK-3β and PTEN expression as compared to other groups. Piroxicam and c-phycocyanin treatment resulted significant apoptotic cell death while showing low PI3-K and Akt expressions. Mitochondrial membrane potential (ΔΨ(M)) alterations (examined by JC-1 and rhodamine 123 labeling of colonocytes) and fluorescence intensity mea...Continue Reading

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Citations

May 28, 2014·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Manpreet Kaur Saini, Sankar Nath Sanyal
Sep 17, 2013·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Katsuhiro TakedaHidemi Kurihara
Mar 19, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Veronica D'AnnunzioJuan J Poderoso
Jun 14, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Qian LiuYu Cai
Jun 4, 2013·Asian Pacific Journal of Cancer Prevention : APJCP·Ashok Kumar Pandurangan
Sep 6, 2020·Molecular Neurobiology·Md Tanvir KabirMohamed M Abdel-Daim
Apr 5, 2016·Asian Pacific Journal of Cancer Prevention : APJCP·Elsayed I SalimHager M Helmy
Oct 2, 2021·ACS Chemical Biology·Maria Rafaele O B da SilvaDaniela de A V Marques

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