Pterostilbene induces mitochondrially derived apoptosis in breast cancer cells in vitro

The Journal of Surgical Research
Dora MoonDavid W McFadden

Abstract

The ability of a breast cancer cell to evade apoptosis has a key role in tumor progression and sensitivity to treatment. High levels of Bcl-2-associated X protein (Bax) in tumor cells have been found to promote apoptosis and sensitize cells to anti-cancer therapies. Bcl-2-associated X protein redistribution to the mitochondrial membrane results in the release of proapoptotic factors including cytochrome C, second-mitochondrial-derived activator of caspase/direct inhibitor of apoptosis-binding protein with low PI (Smac/DIABLO), and Ca(2+). We aimed to explore this pathway in cancerous breast cell lines treated with the naturally occurring antioxidant 3,5-dimethoxy-4-hydroxystilbene (pterostilbene). We used whole cell lysates +/- Bax SiRNA from the cell lines MCF-7 and MDA-MB-231 in an enzyme-linked immunosorbent assay to quantify Bax, cytochrome C, Smac/DIABLO expression, and manganese superoxide dismutase (MnSOD) activity after treatment with pterostilbene. We quantified cell death using histone-related DNA complexes from cytosolic and mitochondrial fractions and used methylthiazol tetrazolium assay to analyze cell proliferation, in the presence of Bax-silencing or scrambled RNA. We measured changes in cytosolic calcium using t...Continue Reading

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Citations

May 22, 2013·Oxidative Medicine and Cellular Longevity·Denise McCormack, David McFadden
Jan 7, 2016·Journal of Cancer Prevention·Eun-Yi Ko, Aree Moon
Jan 23, 2016·Oxidative Medicine and Cellular Longevity·J Antoni SirerolAngel L Ortega
Aug 1, 2015·Nigerian Medical Journal : Journal of the Nigeria Medical Association·Gbenga Anthony AdefolajuMargot Jill Hosie
Sep 6, 2015·Toxicology and Applied Pharmacology·Paul R van GinkelJason D Kenealey
Jan 15, 2014·British Journal of Pharmacology·Elango BhakkiyalakshmiKunka Mohanram Ramkumar
Jul 3, 2013·Critical Reviews in Clinical Laboratory Sciences·José M EstrelaMiguel Asensi
Jan 24, 2016·Biochimica Et Biophysica Acta·Marcos Roberto de OliveiraSeyed Mohammad Nabavi
Aug 25, 2016·Nutrients·Yue ZhouHua-Bin Li
Dec 12, 2019·Critical Reviews in Food Science and Nutrition·Dinh-Chuong PhamBharath Kumar Velmurugan
Apr 24, 2020·Anti-cancer Agents in Medicinal Chemistry·Salwa M El-HalloutyMourad A M Aboul-Soud
Dec 11, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zlatko Dembic
Sep 28, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Paramita Basu, Camelia Maier
Jul 6, 2021·European Journal of Medicinal Chemistry·Ting WangJianyou Shi
Oct 6, 2021·Clinical & Translational Oncology : Official Publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·A HazafaM Naeem

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