PTH (1-34) and growth hormone in prevention of disuse osteopenia and sarcopenia in rats

Bone
Mikkel Bo BrentJesper Skovhus Thomsen

Abstract

Osteopenia and sarcopenia develops rapidly during disuse. The study investigated whether intermittent parathyroid hormone (1-34) (PTH) and growth hormone (GH) administered alone or in combination could prevent or mitigate disuse osteopenia and sarcopenia in rats. Disuse was achieved by injecting 4IU botulinum toxin A (BTX) into the right hindlimb musculature of 12-14-week-old female Wistar rats. Seventy-two rats were divided into six groups: 1. Baseline; 2. Ctrl; 3. BTX; 4. BTX+GH; 5. BTX+PTH; 6. BTX+PTH+GH. PTH (1-34) (60μg/kg/day) and GH (5mg/kg/day). The animals were sacrificed after 6weeks of treatment. Sarcopenia was established by histomorphometry, while the skeletal properties were determined using DXA, μCT, mechanical testing, and dynamic bone histomorphometry. Disuse resulted in lower muscle mass (-63%, p<0.05), trabecular BV/TV (-28%, p<0.05), Tb.Th (-11%, p<0.05), lower diaphyseal cortical thickness (-10%, p<0.001), and lower bone strength at the distal femoral metaphysis (-27%, p<0.001) compared to Ctrl animals. PTH fully counteracted the immobilization-induced lower BV/TV, Tb.Th, and distal femoral metaphyseal strength. GH increased muscle mass (+17%, p<0.05) compared to BTX, but did not prevent the immobilization-...Continue Reading

Citations

Aug 18, 2020·Systematic Reviews·Mikkel Bo BrentJesper Skovhus Thomsen
Jan 4, 2020·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·Yasir ArfatCharles H Hocart
Jan 3, 2021·Calcified Tissue International·Mikkel Bo BrentJesper Skovhus Thomsen
Nov 10, 2020·Frontiers in Endocrinology·Mikkel Bo BrentAnnemarie Brüel
Mar 27, 2021·High Altitude Medicine & Biology·Frederik Duch BromerCasper Bindzus Foldager
May 7, 2021·Frontiers in Endocrinology·Mikkel Bo BrentJesper Skovhus Thomsen
Aug 9, 2021·European Journal of Pharmacology·Mikkel Bo Brent

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