PTHrP inhibits adipocyte differentiation by down-regulating PPAR gamma activity via a MAPK-dependent pathway

Endocrinology
G K ChanA C Karaplis

Abstract

We examined the capacity of PTHrP to modulate the terminal differentiation of the preadipocytic cell line, 3T3-L1. These cells express endogenous PTHrP and its receptor, but expression levels were undetectable after differentiation into mature adipocytes. Cells stably overexpressing PTHrP failed to differentiate when induced to undergo adipogenesis and proliferated at a faster rate. MAPK activity was elevated in PTHrP-transfected 3T3-L1 cells, and treatment with the PKA inhibitor H-8 decreased this activity. Inhibition of MAPK kinase with PD098059 permitted terminal differentiation of PTHrP-transfected 3T3-L1 cells to proceed. Although PPAR gamma gene expression levels remained relatively constant in the PTHrP-transfected cells, PPAR gamma phosphorylation was enhanced. Furthermore, the capacity of PPAR gamma to stimulate transcription in the presence of troglitazone was diminished by PTHrP. Expression of the PPAR gamma-regulated adipocyte specific gene aP2 transiently rose and then fell in PTHrP-transfected cells. These results indicate that PTHrP can increase MAPK activity in 3T3-L1 cells via the PKA pathway, thereby enhancing PPAR gamma phosphorylation. This modification can inactivate the transcriptional enhancing activity o...Continue Reading

Citations

Aug 5, 2010·Physiological Genomics·Siddharth SukumaranRichard R Almon
Mar 11, 2008·Metabolism: Clinical and Experimental·Vanessa A ConstantAlexander Sorisky
Nov 27, 2004·Biology of Reproduction·Nandini BhattacharyaKwan Hee Kim
Feb 7, 2017·Cell Metabolism·Yi FanBeate Lanske
Aug 26, 2003·Journal of Cellular Biochemistry·Elizabeth H AllanT John Martin
Apr 23, 2011·Thyroid : Official Journal of the American Thyroid Association·Tereza PlanckMikael Lantz
Nov 2, 2007·Journal of Cellular Biochemistry·Pablo AstudilloJ Pablo Rodríguez
Sep 10, 2008·Current Osteoporosis Reports·Stavroula Kousteni, John P Bilezikian
Feb 12, 2009·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·Z C Dang
Jun 8, 2019·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Mengyu YangToshihide Mizoguchi
May 9, 2014·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Partha SinhaJoy Y Wu
May 18, 2004·Arthritis and Rheumatism·Saifeddin AlsalamehMartin Lotz
Feb 3, 2015·Journal of Cellular Biochemistry·Oscar DonosoJ Pablo Rodríguez
Sep 11, 2019·Journal of Hematology & Oncology·Qi WuShengrong Sun
May 25, 2004·Journal of Cellular Biochemistry·Zhi-Chao Dang, Clemens W G M Lowik
Jan 9, 2016·Climacteric : the Journal of the International Menopause Society·Y YangJ Shen
Feb 24, 2005·Molecular and Cellular Biochemistry·Z C Dang, C W G M Lowik
Jul 18, 2018·Frontiers in Endocrinology·Guojing LuoXijie Yu
Jun 17, 2003·The Journal of Biological Chemistry·Amelia J EntinghC Ronald Kahn
Nov 8, 2002·The Journal of Biological Chemistry·Zhi-Chao DangClemens W G M Löwik
Nov 6, 2020·Nature Reviews. Endocrinology·Eben G Estell, Clifford J Rosen
Feb 17, 2021·Clinical & Experimental Metastasis·Sneha SoniChandi C Mandal
Feb 8, 2005·Biochemical and Biophysical Research Communications·Robert C GensureHarald Jüppner
Jul 3, 2021·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Toshihide Mizoguchi, Noriaki Ono

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.