PTHrP isoforms have differing effect on chondrogenic differentiation and hypertrophy of mesenchymal stem cells

Biochemical and Biophysical Research Communications
Jong-Min Lee, Gun-Il Im

Abstract

While several isoforms of parathyroid hormone-related peptide (PTHrP) have been commercially available, the difference in their effect has not been widely studied. The purpose of this study was to determine which isoform most effectively promoted chondrogenesis and suppressed hypertrophy from mesenchymal stem cells (MSCs). MSCs isolated from fresh bone marrow were cultured in pellet in chondrogenic medium containing 5 ng/ml of transforming growth factor (TGF)-β(3). From day 14 of culture, subsets of pellets were additionally treated with one of the four PTHrP isoforms (1-34, 1-86, 7-34, and 107-139) at 100 nM. After a further 2 weeks of in vitro culture, pellets were harvested for analysis. PTHrPs 1-34 and 1-86 significantly decreased the DNA level (p<0.05) while PTHrPs 7-34 and 107-139 significantly increased DNA level (p<0.05) compared with the control treated with TGF-β(3) only. Glycosaminoglycan per DNA significantly increased when treated with PTHrPs 1-34 and 1-86 (p<0.05) while it significantly decreased with PTHrPs 7-34 and 107-139 (p<0.05). PTHrP 1-34 significantly increased the gene and protein expression of the chondrogenic marker COL2A1, and decreased those of hypertrophic markers COL10A1 and alkaline phosphatase whi...Continue Reading

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Citations

Aug 20, 2015·International Journal of Molecular Sciences·Leilei ZhongJanine N Post
Sep 21, 2013·Gene Expression Patterns : GEP·Anna CzarkwianiPaola Oliveri
Aug 19, 2016·Biotechnology Letters·Ji-Yun Ko, Gun-Il Im
Oct 27, 2017·Biotechnology Journal·Howard C TribeRahul S Tare
Sep 8, 2018·Journal of Cellular Biochemistry·Sanam DolatiAhmad Reza Jodati
Mar 8, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Jong Min LeeGun-Il Im
Jun 14, 2017·Pharmaceuticals·Bethanie I AyerstAnthony J Day
Apr 3, 2020·International Journal of Molecular Sciences·Yeri Alice RimJi Hyeon Ju
Jul 7, 2020·Stem Cells·Jung Woo ParkRen-He Xu
Feb 2, 2021·Frontiers in Bioengineering and Biotechnology·Adelheid WeidingerAsmita Banerjee
Jan 15, 2021·Cancers·Xavier Ruiz-PlazasMatilde R Chacón

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