PTOV1: a novel testosterone-induced atherogenic gene in human aorta

The Journal of Pathology
Y NakamuraH Sasano

Abstract

There are gender differences in the development of atherosclerosis, possibly owing to differences in sex steroid hormone action and/or metabolism. One of the atherogenic effects of testosterone is thought to be androgen receptor (AR)-mediated vascular smooth muscle cell (VSMC) proliferation. However, the detailed mechanism of this effect, particularly the identity of the genes associated with VSMC proliferation, remains largely unknown. Therefore, we first employed microarray analysis and, subsequently, quantitative RT-PCR to analyse RNA expression in AR-positive human VSMCs treated with testosterone in order to detect testosterone-induced genes associated with cell proliferation. We further examined whether the genes identified were involved in cell proliferation using small interfering RNA (siRNA) transfection. Expression of the gene products was then evaluated in human aorta with various degrees of atherosclerosis in order to evaluate the clinical relevance of the findings. Both microarray and quantitative RT-PCR analyses demonstrated marked induction of the human prostate overexpressed protein 1 (PTOV1) gene by testosterone in the cell lines: this gene was recently identified as a novel androgen-induced gene involved in pro...Continue Reading

References

Feb 1, 1983·American Journal of Epidemiology·D L WingardE Barrett-Connor
May 1, 1995·Arteriosclerosis, Thrombosis, and Vascular Biology·M R AdamsJ R Kaplan
Nov 14, 1997·Arteriosclerosis, Thrombosis, and Vascular Biology·S M HermanD S Celermajer
Feb 24, 2001·Menopause : the Journal of the North American Menopause Society·G P BerniniA Salvetti
Jan 15, 2002·The Journal of Clinical Endocrinology and Metabolism·Maro R I WilliamsPaul A Komesaroff
Jun 6, 2003·Endocrine Reviews·Peter Y LiuDavid J Handelsman
May 20, 2004·Molecular and Cellular Endocrinology·Yasuhiro NakamuraHironobu Sasano
Jul 14, 2004·European Journal of Endocrinology·Richard D JonesKevin S Channer
Dec 8, 2004·The American Journal of Pathology·Yasuhiro NakamuraHironobu Sasano
Feb 17, 2005·Molecular and Cellular Biology·Anna SantamaríaRosanna Paciucci

❮ Previous
Next ❯

Citations

Apr 4, 2013·The Journal of Endocrinology·Daniel M Kelly, T Hugh Jones
Jun 6, 2012·Molecular and Cellular Endocrinology·Kondababu KurakulaCarlie J M de Vries
Nov 3, 2015·Journal of Atherosclerosis and Thrombosis·Sumiko YoshidaKen-Ichi Aihara
Dec 30, 2014·Molecular Aspects of Medicine·Alessandra di MasiClara Nervi
Jun 28, 2017·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Rong LiCan-Xia Xu
Dec 29, 2016·Oncotarget·Verónica CánovasRosanna Paciucci
May 3, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Juan MoroteInés M de Torres
Jul 23, 2021·Cardiovascular Research·Antonio CittadiniAndrea Salzano

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.