PtrMYB57 contributes to the negative regulation of anthocyanin and proanthocyanidin biosynthesis in poplar

Plant Cell Reports
Shuzhen WanKeming Luo

Abstract

A novel R2R3 MYB transcription factor PtrMYB57 interacted with bHLH131 and PtrTTG1 to form the MBW complex and negatively regulated the biosynthesis of both anthocyanins and PAs in poplar. R2R3-MYB transcription factors (TFs) are important regulators of secondary metabolite biosynthesis in woody species. A series of R2R3-MYB TFs involved in anthocyanin and proanthocyanidin (PA) biosynthesis have been identified in poplar. In this study, we report the identification and characterization of a subgroup 4 MYB member PtrMYB57, which contains a repressor domain (LxLxL) at the C-terminal end. PtrMYB57 encodes an R2R3 MYB protein localized in the nucleus and is predominantly expressed in mature leaves. Transgenic poplar overexpressing PtrMYB57 showed a reduction in anthocyanin and PA accumulation compared to wild-type plants. By contrast, a high anthocyanin and PA phenotype was observed in Ptrmyb57 mutants generated by the CRISPR/Cas9 system. Furthermore, transient expression assays revealed that PtrMYB57 interacted with bHLH131 (bHLH) and PtrTTG1 (WDR) to form the MBW complex and bound to the flavonoid gene promoters, leading to inhibition of these promoters. Taken together, our results suggest that PtrMYB57 plays a negative role in t...Continue Reading

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Citations

Sep 16, 2018·The Plant Journal : for Cell and Molecular Biology·Di FanKeming Luo
May 16, 2020·International Journal of Molecular Sciences·Ying DaiHua Cassan-Wang
Jul 16, 2020·International Journal of Molecular Sciences·Hainan Tian, Shucai Wang
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Dec 12, 2018·Frontiers in Plant Science·William Patrick BewgChung-Jui Tsai
Aug 15, 2020·Frontiers in Plant Science·Erika N DortRichard C Hamelin
Nov 7, 2019·International Journal of Molecular Sciences·Muhammad AnwarLihui Zeng
Jan 22, 2021·Plant Cell Reports·Dragana MiladinovicZoe Hilioti
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Apr 4, 2021·International Journal of Molecular Sciences·Huiling YanXiyang Zhao
Apr 4, 2021·Plants·Inger Bæksted HolmeHenrik Brinch-Pedersen
Aug 28, 2021·International Journal of Molecular Sciences·Emil KhusnutdinovElena Mikhaylova

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Methods Mentioned

BETA
environmental stresses
transgenic
PCR
two-hybrid
confocal microscopy
one-hybrid

Software Mentioned

MEGA6
DNAMAN
DNAMAN8
MEGA5

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