Ptychographic X-ray nanotomography quantifies mineral distributions in human dentine

Scientific Reports
I ZanetteP Zaslansky

Abstract

Bones are bio-composites with biologically tunable mechanical properties, where a polymer matrix of nanofibrillar collagen is reinforced by apatite mineral crystals. Some bones, such as antler, form and change rapidly, while other bone tissues, such as human tooth dentine, develop slowly and maintain constant composition and architecture for entire lifetimes. When studying apatite mineral microarchitecture, mineral distributions or mineralization activity of bone-forming cells, representative samples of tissue are best studied at submicrometre resolution while minimizing sample-preparation damage. Here, we demonstrate the power of ptychographic X-ray tomography to map variations in the mineral content distribution in three dimensions and at the nanometre scale. Using this non-destructive method, we observe nanostructures surrounding hollow tracts that exist in human dentine forming dentinal tubules. We reveal unprecedented quantitative details of the ultrastructure clearly revealing the spatially varying mineralization density. Such information is essential for understanding a variety of natural and therapeutic effects for example in bone tissue healing and ageing.

References

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Citations

Jul 18, 2016·Dental Materials : Official Publication of the Academy of Dental Materials·Christoph JudFranz Pfeiffer
Jun 3, 2017·The Review of Scientific Instruments·Tomaš StankevičStefan Kubsky
Sep 2, 2017·Journal of Synchrotron Radiation·R D FreeD Joester
Mar 11, 2020·Journal of Synchrotron Radiation·T S ArgunovaE D Nazarova
Jul 8, 2017·Scientific Reports·Marcus Gallagher-JonesJianwei Miao
Jan 14, 2021·Proceedings of the National Academy of Sciences of the United States of America·Yuan Hung LoJianwei Miao
Feb 8, 2021·Journal of the Mechanical Behavior of Biomedical Materials·Elsa VennatDenis Aubry

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Methods Mentioned

BETA
X-ray
electron microscopy
of density
scanning electron microscopy

Software Mentioned

PXCT

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