Pubertal down-regulation of Tetraspanin 8 in testicular Sertoli cells is crucial for male fertility.

Molecular Human Reproduction
Bhola Shankar PradhanSubeer S Majumdar

Abstract

The alarming decline in sperm count has become a global concern in the recent decades. The division and differentiation of male germ cells (Gc) into sperm are governed by Sertoli cells (Sc) upon their functional maturation during puberty. However, the roles of genes regulating pubertal maturation of Sc have not been fully determined. We have observed that Tetraspanin 8 (Tspan8) is down-regulated in Sc during puberty in rats. However, there has been no in vivo evidence for a causal link between the down-regulation of Tspan8 expression and the onset of spermatogenesis as yet. To investigate this, we generated a novel transgenic (Tg) rat, in which the natural down-regulation of Tspan8 was prevented specifically in Sc from puberty up to adulthood. Adult Tg male rats showed around 98% reduction in sperm count despite having a similar level of serum testosterone (T) as the controls. Functional maturation of Sc was impaired as indicated by elevated levels of Amh and low levels of Kitlg and Claudin11 transcripts. The integrity of the blood testis barrier was compromised due to poor expression of Gja1 and Gc apoptosis was discernible. This effect was due to a significant rise in both Mmp7 and phospho P38 MAPK in Tg rat testis. Taken tog...Continue Reading

References

Jul 15, 1998·Human Reproduction·D S Irvine
Nov 14, 1998·Seminars in Cell & Developmental Biology·M D Griswold
Feb 24, 1999·Lancet·J TesarikC Mendoza
May 30, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·Richard M SharpeJane S Fisher
Oct 22, 2004·Tissue Antigens·M D WrightA B van Spriel
Nov 13, 2004·Human Reproduction·Sheba JarvisCarol Readhead
Feb 3, 2005·Nature Reviews. Immunology·Shoshana Levy, Tsipi Shoham
Nov 30, 2005·Nature Reviews. Molecular Cell Biology·Martin E Hemler
Dec 22, 2005·The Journal of Clinical Endocrinology and Metabolism·Yendrembam Sangeeta DeviSubeer S Majumdar
Dec 22, 2006·Traffic·Fedor Berditchevski, Elena Odintsova
Jun 27, 2007·The American Journal of Pathology·Ralph BrehmMartin Bergmann
Mar 28, 2008·Clinical & Experimental Metastasis·Zhuan ZhouZhi-Hua Yang
Sep 5, 2008·Biology of Reproduction·Michelle A Wood, William H Walker
Nov 8, 2008·Nature Medicine·Martin M Matzuk, Dolores J Lamb
May 12, 2009·The Biochemical Journal·Stéphanie CharrinEric Rubinstein
Sep 11, 2009·Biology of Reproduction·S Mazaud-GuittotB Le Magueresse-Battistoni
Nov 3, 2009·Journal of Reproductive Immunology·Subeer S MajumdarSuveera Dhup
Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Ilona A KoperaDolores D Mruk
Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Carla M K MorrowRex A Hess
Jun 21, 2011·The Journal of Urology·John W WeedinLarry I Lipshultz
Jun 21, 2011·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Anna HentrichLutz Konrad
Sep 10, 2011·Science·Richard M Sharpe
Oct 20, 2011·Nature Reviews. Endocrinology·Claire BouvattierJacques Young
Aug 2, 2012·American Journal of Physiology. Endocrinology and Metabolism·Indrashis BhattacharyaSubeer S Majumdar
Feb 5, 2013·Progress in Biophysics and Molecular Biology·Subeer S Majumdar, Indrashis Bhattacharya
Jul 23, 2013·American Journal of Reproductive Immunology : AJRI·Agnieszka MalcherMaciej Kurpisz
Sep 5, 2013·Molecular and Cellular Biochemistry·Xianghong WuLaval Andy Alain
Dec 7, 2013·Scientific Reports·Abul UsmaniSubeer S Majumdar
Sep 14, 2014·Journal of Cell Science·Ka-Wai MokC Yan Cheng
Apr 26, 2016·Molecular Therapy. Nucleic Acids·Bhola Shankar Pradhan, Subeer S Majumdar
May 4, 2016·Cell and Tissue Research·Indrashis BhattacharyaSubeer S Majumdar
Oct 14, 2016·Biology of Reproduction·Kazadi MutojiBrian P Hermann
Jan 10, 2017·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Kamal MandalSubeer S Majumdar
Jan 11, 2017·PloS One·Sandip MukherjeeSamir Bhattacharya

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