Abstract
Saponin is an active component of many phytomedicine, which has extensive pharmacology effects. Meanwhile, it is reported that cytotoxicity, especially hemolysis and hepatotoxicity, in pentacyclic triterpenoid saponin (PTS) hindered their further development and application. Surface activity, a unique physical property of saponins, is believed to be related to membrane toxicity. However, the correlation between the surface activity and cytotoxicity of saponins is still unexplained. In this paper, our aim was to explore the relationship between surface activity-cytotoxicity of pulchinenosides and the hepatotoxicity mechanism of PTS in vitro. The surface activity of different saponins was investigated by contact angle, surface free energy (SFE), and oil/water partition coefficient (log Papp). In the cytotoxicity study, the hemolysis and hepatotoxicity activity of different saponins was compared by HD50 of erythrocyte and MTT, flow cytometry and LDH assay in LO2 cells respectively. And in the hepatotoxicity mechanism study, western blot was used for observing the expression of proteins related to apoptosis and exploring the liver injury mechanism of PTS. The results suggested that the influences of surface activity on hepatocytes ...Continue Reading
References
Jan 1, 1975·Biochemical Pharmacology·R Segal, I Milo-Goldzweig
May 30, 1974·Molecular and Cellular Biochemistry·S ShanyK S Kim
Nov 1, 1981·Journal of Pharmacobio-dynamics·J HaseT Tomimori
Mar 22, 2000·Acta histochemica·E BaumannW Linss
Dec 21, 2002·The British Journal of Nutrition·George FrancisKlaus Becker
Feb 5, 2003·Cancer Letters·Janaina FernandesCerli Rocha Gattass
Mar 20, 2003·Journal of Agricultural and Food Chemistry·Uppuluri V MallavadhaniC Manjula
Jul 5, 2003·Biological & Pharmaceutical Bulletin·Junzo NakamuraKoyo Nishida
Apr 20, 2006·Journal of Ethnopharmacology·Norihiro BannoTakashi Suzuki
Feb 20, 2007·Bioorganic & Medicinal Chemistry·Yibing WangBiao Yu
Sep 14, 2010·Phytochemistry Reviews : Proceedings of the Phytochemical Society of Europe·Irma PodolakDanuta Sobolewska
Feb 22, 2011·Phytochemistry·Jörg M AugustinSøren Bak
Apr 17, 2012·Bioorganic & Medicinal Chemistry·Stefan BöttgerMatthias F Melzig
Sep 4, 2012·Toxicology Letters·Xiaoming ZhaoJinyong Peng
Sep 28, 2012·Beilstein Journal of Organic Chemistry·Elena A KhatuntsevaNikolay E Nifantiev
Sep 3, 2013·Chemico-biological Interactions·Li ChenShizhong Zheng
Nov 26, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Yali LiuShilin Yang
Feb 5, 2014·International Immunopharmacology·Hao YuJinyong Peng
Sep 27, 2014·Fitoterapia·Dan SuYulin Feng
Oct 9, 2014·Organic & Biomolecular Chemistry·Joseph H LorentMarie-Paule Mingeot-Leclercq
Nov 22, 2015·Environmental Toxicology·Shuli ManWenyuan Gao
Nov 24, 2018·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Dan-Qian ChenYing-Yong Zhao
Feb 1, 2019·Journal of Ethnopharmacology·Yonggui SongDan Su
Apr 9, 2019·Journal of Ethnopharmacology·Haiyin ZhengWei Xu