Pulchinenosides from Pulsatilla Chinensis Increase P-Glycoprotein Activity and Induce P-Glycoprotein Expression

Evidence-based Complementary and Alternative Medicine : ECAM
Yali LiuPhillip M Gerk

Abstract

Five pulchinenosides (pulchinenoside B3, pulchinenoside BD, pulchinenoside B7, pulchinenoside B10, and pulchinenoside B11) isolated from Pulsatilla chinensis (Bge) Regel saponins extract exhibited strong antitumor activities but poor gastrointestinal absorption properties. The enteric induction of P-glycoprotein (P-gp) is understood to restrict the oral bioavailability of some pharmaceutical compounds and lead to adverse drug reactions. Therefore, the present investigation was intended to delineate the impacts of pulchinenosides on cellular P-gp function and expression using Sf9 membrane vesicles and LS180 cells as a surrogate of human intestinal epithelial cells. Preliminary cytotoxic studies showed that 10 μM was an acceptable concentration for cytotoxicity and antiproliferation studies for all pulchinenosides using the alamarBlue assay. The cell cycle of LS180 cells detected by flow cytometry was not significantly influenced after 48 hours of coincubation with 10 μM of pulchinenosides. In the presence of pulchinenosides, the ATP-dependent transport of N-methyl-quinidine mediated by P-glycoprotein was stimulated significantly. The upregulation of P-glycoprotein and mRNA levels was found by Western blot and real-time PCR analy...Continue Reading

References

Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·F ThiebautM C Willingham
Apr 21, 1993·Journal of the National Cancer Institute·P M Chaudhary, I B Roninson
Oct 31, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·L HuangM Vore
Dec 10, 1999·Journal of Clinical Pharmacology·D K Yu
Feb 22, 2000·Lancet·F RuschitzkaG Noll
Jan 11, 2001·European Journal of Clinical Pharmacology·E Ernst
Apr 12, 2001·The Journal of Biological Chemistry·A GeickO Burk
Nov 25, 2003·Gut·C G DietrichR P J Oude Elferink
Nov 8, 2005·Chemical & Pharmaceutical Bulletin·Seong-Cheol BangByung-Zun Ahn
May 6, 2006·Biochemical Pharmacology·Yi HanLee-Yong Lim
Nov 17, 2006·The Journal of Pharmacology and Experimental Therapeutics·Phillip M GerkMary Vore
Apr 25, 2007·Critical Reviews in Food Science and Nutrition·Ozlem Güçlü-Ustündağ, Giuseppe Mazza
Jun 11, 2008·Journal of Agricultural and Food Chemistry·Hideo SatsuMakoto Shimizu
Aug 1, 2008·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·S-F Zhou
Aug 20, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Alaa H AbuznaitAmal Kaddoumi
Oct 18, 2011·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Qiong-ming XuXiao-ran Li
Dec 19, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Zsombor MiskolczyCatherine Amiel
Oct 31, 2019·Evidence-based Complementary and Alternative Medicine : ECAM·Ling ZhangDan Su

❮ Previous
Next ❯

Methods Mentioned

BETA
scraping
flow cytometry
PCR

Software Mentioned

Gen5
QUANT
NIH ImageJ
GraphPad
STUDIO
GraphPad Prism
Prism

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.