Pulling the chromatin

The European Physical Journal. E, Soft Matter
C Claudet, J Bednar

Abstract

Nucleosome is the basic subunit of the chromatin, which organizes the genomic DNA within the cell nucleus. It was understood in the last decade that beside the DNA compaction it plays an important role in the regulation of the gene expression. In its intact form, the nucleosome represents an important mechanical barrier and, among others, it prevents access to the DNA and blocks the transcription elongation. Therefore, it has become important to know the forces and energies necessary to destabilize the nucleosome in order to understand the DNA-related processes. Stretching the chromatin fibre using micromanipulation techniques (e.g. optical tweezers) is an ideal approach to study the nucleosomal stability and the parameters that can modify it. In this short review we will discuss the existing data and potential difficulties that this state-of-the-art technique still has to overcome.

References

Sep 25, 1979·Nucleic Acids Research·D M LilleyM Houghton
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G ArentsE N Moudrianakis
Jul 5, 1991·Journal of Molecular Biology·S PenningsE M Bradbury
Mar 7, 1989·Biochemistry·T D YagerK E van Holde
Oct 11, 1984·Nature·T J RichmondA Klug
Jan 24, 1981·Nucleic Acids Research·R W Cotton, B A Hamkalo
May 1, 1995·Journal of Structural Biology·P FurrerA Prunell
Oct 4, 1996·The Journal of Biological Chemistry·T ItoJ T Kadonaga
Oct 23, 1997·Biophysical Journal·J F Marko, E D Siggia
Apr 16, 1998·The Journal of Biological Chemistry·G A McQuibbanP N Lewis
Apr 16, 1998·Genes & Development·K Struhl
Sep 30, 1998·Methods in Enzymology·K Visscher, S M Block
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·J BednarC L Woodcock
Jan 5, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y Cui, C Bustamante
Feb 25, 2000·Journal of Molecular Biology·J D Anderson, J Widom
Dec 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·B LadouxJ L Viovy
Dec 29, 2000·Nature·T MisteliD T Brown
May 8, 2001·Annual Review of Biophysics and Biomolecular Structure·E Evans
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Brent D Brower-TolandMichelle D Wang
Jan 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sanford H LeubaJordanka Zlatanova
Aug 5, 2003·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·John F Marko, Michael G Poirier
Jul 13, 2004·Physical Review Letters·I M Kulić, H Schiessel
Jul 23, 2004·The Journal of Biological Chemistry·Dimitar AngelovStefan Dimitrov
Nov 30, 2004·Science·Benedetta DorigoTimothy J Richmond
Jan 12, 2005·Current Topics in Developmental Biology·Corey L Smith, Craig L Peterson
Jan 25, 2005·Journal of Molecular Biology·Brent Brower-TolandMichelle D Wang
Feb 25, 2005·Proceedings of the National Academy of Sciences of the United States of America·Miroslav TomschikSanford H Leuba
Mar 18, 2005·The Journal of Biological Chemistry·Cyril ClaudetJan Bednar
Jan 1, 1986·Biophysical Journal·K O GreulichH Eisenberg

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Citations

May 8, 2008·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Sanford H LeubaSaleem A Khan
Mar 8, 2006·The European Physical Journal. E, Soft Matter·A Benecke, UNKNOWN Systems Epigenomics Group
May 19, 2010·International Journal of Molecular Sciences·Christophe LavelleJordanka Zlatanova
May 28, 2009·Biochemical and Biophysical Research Communications·Feroz M HameedG V Shivashankar

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