Pullulan Nanoparticles as Prebiotics Enhance the Antibacterial Properties of Lactobacillus plantarum Through the Induction of Mild Stress in Probiotics

Frontiers in Microbiology
Liang HongChong-Su Cho

Abstract

Synbiotics, which are the combination of probiotics and prebiotics, have recently attracted attention because of their synergistic net health benefits. Probiotics have been used as alternatives to antibiotics. Among the probiotics, Lactobacillus plantarum (LP) has shown strong antimicrobial activity against Escherichia coli K99, a major livestock pathogen. In this study, we aimed to investigate the antimicrobial activity of phthalyl pullulan nanoparticle (PPN)-treated LP. Interestingly, when PPNs were added to LP, the PPNs were internalized into the LP through an energy-dependent and galactose transporter-dependent mechanism. Additionally, more plantaricin, a natural antibacterial peptide, was secreted from PPN-treated LP than from untreated or pullulan-treated LP. Furthermore, antimicrobial activity against Gram-negative Escherichia coli K99 and Gram-positive Listeria monocytogenes by PPN-treated LP was higher than those of untreated or pullulan-treated LP. It is thought that the enhanced antimicrobial properties of the PPN-treated LP are due to intracellular stimulation. Overall, this research provides a new method of producing plantaricin in LP through intracellular stimulation by internalized PPNs.

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Citations

Mar 22, 2020·Journal of Functional Biomaterials·Maria-Beatrice ColtelliPierfrancesco Morganti
Apr 28, 2020·IET Nanobiotechnology·Parveen Kaur Sidhu, Kiran Nehra
Apr 1, 2020·International Journal of Molecular Sciences·Alessandra DurazzoAntonello Santini
Jul 1, 2020·Probiotics and Antimicrobial Proteins·Rafig GurbanovGizem Samgane
Dec 29, 2020·Journal of Applied Microbiology·R Sulthana, A C Archer
Sep 4, 2021·Frontiers in Microbiology·Liang HongChong-Su Cho

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Methods Mentioned

BETA
dynamic light scattering
electrophoretic light scattering
flow cytometry
Assay
electrophoresis
PCR
fluorescence-activated cell sorting
FACS

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