PMID: 3769565Nov 1, 1986Paper

Pulmonary alveolar proteinosis. Further evaluation of abnormal alveolar macrophages

Chest
R J Gonzalez-Rothi, J O Harris

Abstract

To investigate the function of alveolar macrophages (AM) and the mechanisms of impairment in pulmonary alveolar proteinosis, we established in culture AM from three patients and from eight normal nonsmokers and assessed phagocytosis and phagolysosome fusion by the acridine orange assay with live yeast as the phagocytic challenge. Alveolar macrophages from the patients with pulmonary alveolar proteinosis ingested fewer yeasts per cell than did normal AM (mean +/- SE, 2.3 +/- 0.3 vs 3.3 +/- 0.2; p less than 0.05) and had decreased phagolysosome fusion (33 +/- 6 percent vs 64 +/- 1 percent; p less than 0.001). Alveolar macrophages from three normal subjects were incubated with cell-free fractions isolated by centrifugation of lavage fluid from the patients at 250 g (P1) or centrifugation of P1 supernatant at 20,000 g (P2). The P1 fraction did not decrease the number of AM ingesting yeast or the number of yeast cells ingested per cell, but the P2 fraction decreased both phagocytic indices. Conversely, phagolysosome fusion was depressed by the P1 fraction (48 +/- 3 percent vs 66 +/- 2 percent for untreated AM from the same subject; p less than 0.02) but not by the P2 fraction. Significant morphologic changes were noted in AM cocultu...Continue Reading

References

Sep 1, 1976·Annals of Internal Medicine·D W GoldeM J Cline
Jan 1, 1969·British Journal of Diseases of the Chest·J M Davidson, W M Macleod
Dec 1, 1969·American Journal of Diseases of Children·J A Danigelis, B Markarian
Sep 1, 1984·Journal of Leukocyte Biology·M B GorenC Miranti
Jun 5, 1958·The New England Journal of Medicine·S H ROSENA A LIEBOW
Jun 1, 1960·Annals of Internal Medicine·W FRAIMOWR C TAYLOR

❮ Previous
Next ❯

Citations

Nov 22, 1997·Pediatric Pulmonology·R V NachajonM H Collins
Jul 12, 2002·American Journal of Hematology·Akira TomonariShigetaka Asano
Jul 30, 2011·Rheumatology International·Eugene ParkSang-Heon Lee
Jan 11, 2011·Bone Marrow Transplantation·J PidalaH Fernandez
Dec 26, 2003·The New England Journal of Medicine·Bruce C TrapnellKoh Nakata
Oct 23, 2010·Current Opinion in Allergy and Clinical Immunology·Sarah K Browne, Steven M Holland
Feb 7, 2008·Pathology International·Makoto Naito
Sep 3, 2011·Pathology International·Michiya NishinoRichard L Kradin
Feb 17, 2000·Australian and New Zealand Journal of Medicine·Y C LeeB W Robinson
Apr 1, 1991·Acta paediatrica Scandinavica·K PaulW Nützenadel
Sep 1, 1989·Postgraduate Medical Journal·J PascualL Fogué
Feb 5, 2002·Annual Review of Physiology·Bruce C Trapnell, Jeffrey A Whitsett
Dec 31, 1997·American Journal of Respiratory and Critical Care Medicine·H Q ShenY Suzuki
Apr 14, 2000·American Journal of Respiratory and Critical Care Medicine·M S CarrawayC A Piantadosi
Apr 1, 1989·The American Review of Respiratory Disease·R M HoffmanR M Rogers
Mar 1, 1990·The American Review of Respiratory Disease·J Kelley
Mar 1, 2005·American Journal of Respiratory and Critical Care Medicine·Ryushi TazawaToshihiro Nukiwa
Jul 18, 2002·American Journal of Respiratory and Critical Care Medicine·John F Seymour, Jeffrey J Presneill
Aug 19, 2006·American Journal of Respiratory Cell and Molecular Biology·Pierre-Yves BerclazBruce C Trapnell
Jan 5, 2000·FEBS Letters·M GustafssonJ Johansson
Oct 1, 1990·Chest·A Merino-AnguloR Díaz de Otazu
Sep 1, 1989·Chest·R H Israel, C R Magnussen
Mar 15, 2011·Pediatric Transplantation·Marc AnsariJean-Christophe Fournet
Nov 10, 2012·Arthritis Care & Research·Yukiko KimuraUNKNOWN Childhood Arthritis Rheumatology Research Alliance Carra Net Investigators
Nov 27, 2010·The Lancet Infectious Diseases·Sarah K Browne, Steven M Holland
Mar 20, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lindsey B RosenSarah K Browne
Jan 15, 2016·Antibodies·Vijaya KnightMichael D O'Sullivan
Feb 3, 2004·Critical Reviews in Computed Tomography·Maureen R Horton
Apr 13, 1999·The American Journal of Physiology·J R WrightC I Restrepo
Jul 30, 2019·Frontiers in Immunology·Patricia A MerkelVijaya Knight

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.