Pulmonary dendritic cells isolated from neonatal and adult ovine lung tissue

Veterinary Immunology and Immunopathology
Sasha J FachRandy E Sacco

Abstract

Lung dendritic cells (DCs) are potent antigen presenting cells (APCs) that initiate and modulate the adaptive immune response upon microbial infection within the pulmonary environment. For the first time, neonatal and adult lung DCs in a large animal model were compared in these studies. Here, we isolated and identified lung DCs in both neonatal and adult sheep, a valuable experimental animal utilized in pulmonary studies of naturally occurring respiratory diseases. Neonatal lung DCs exhibited characteristic dendrites and morphology when observed by transmission electron microscopy and expressed low to moderate DEC-205, CD80/86, MHC class II and CD 14. Regardless of age, lung DCs were functionally able to endocytose FITC conjugated ovalbumin but to a lesser degree than monocyte-derived DCs. In addition, neonatal lung DCs were demonstrated to be potent stimulators of allogeneic T cell proliferation. Together, these results demonstrate that neonatal and adult lung DCs are functionally similar. It is apparent from the data presented that neonatal pulmonary DCs do not exhibit an intrinsic functional defect that would impair their ability to take up antigen and stimulate naïve T cells. These data support growing evidence that neonat...Continue Reading

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Citations

Feb 19, 2009·Immunology and Cell Biology·Shanjana AwasthiGary White
Dec 4, 2012·Viruses·Rachel J Derscheid, Mark R Ackermann
Dec 26, 2008·Veterinary Parasitology·M Yavuz GulbaharY Emre Beyhan
Jan 29, 2008·Developmental and Comparative Immunology·Caroline Piercey AkessonMona Aleksandersen
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Dec 29, 2007·Immunological Investigations·W R Waters, R E Sacco
Dec 1, 2017·Frontiers in Immunology·Laura Lambert, Fiona J Culley

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