Abstract
Lung dendritic cells (DCs) are potent antigen presenting cells (APCs) that initiate and modulate the adaptive immune response upon microbial infection within the pulmonary environment. For the first time, neonatal and adult lung DCs in a large animal model were compared in these studies. Here, we isolated and identified lung DCs in both neonatal and adult sheep, a valuable experimental animal utilized in pulmonary studies of naturally occurring respiratory diseases. Neonatal lung DCs exhibited characteristic dendrites and morphology when observed by transmission electron microscopy and expressed low to moderate DEC-205, CD80/86, MHC class II and CD 14. Regardless of age, lung DCs were functionally able to endocytose FITC conjugated ovalbumin but to a lesser degree than monocyte-derived DCs. In addition, neonatal lung DCs were demonstrated to be potent stimulators of allogeneic T cell proliferation. Together, these results demonstrate that neonatal and adult lung DCs are functionally similar. It is apparent from the data presented that neonatal pulmonary DCs do not exhibit an intrinsic functional defect that would impair their ability to take up antigen and stimulate naïve T cells. These data support growing evidence that neonat...Continue Reading
References
Mar 1, 1992·American Journal of Respiratory Cell and Molecular Biology·K M McCarthyE E Schneeberger
Mar 1, 1992·The Journal of Experimental Medicine·J L GongE E Schneeberger
Jun 1, 1991·The Journal of Experimental Medicine·M A Schon-HegradP G Holt
Feb 1, 1986·The Journal of Experimental Medicine·K SertlE M Shevach
Oct 1, 1983·Veterinary Microbiology·H D Lehmkuhl, R C Cutlip
May 1, 1994·American Journal of Respiratory Cell and Molecular Biology·J M van HaarstH C Hoogsteden
Jul 1, 1996·Acta Paediatrica·C LindgrenH W Sundell
Dec 1, 1996·The Journal of Experimental Medicine·A S McWilliamP G Holt
Oct 28, 1999·American Journal of Respiratory Cell and Molecular Biology·L CochandL P Nicod
Dec 30, 1999·Veterinary Immunology and Immunopathology·C J HowardR A Collins
Feb 13, 2001·Infection and Immunity·M Gonzalez-Juarrero, I M Orme
Nov 9, 2002·Immunology·Simon S M ChanBarbara A Blacklaws
Jan 15, 2003·Journal of Leukocyte Biology·Anna A BajerD Mark Estes
Feb 27, 2003·Immunology·Micheline LagranderieBoris B Vargaftig
Mar 11, 2004·Immunology·Daniel R GliddonChristopher J Howard
Apr 22, 2004·Journal of Immunological Methods·Claudia J CalderAndrew D Dick
Jul 2, 2004·Nature Reviews. Immunology·Becky AdkinsStuart Marshall-Clarke
Nov 24, 2004·Microbes and Infection·David K MeyerholzMark R Ackermann
Dec 4, 2004·American Journal of Respiratory Cell and Molecular Biology·Ingel K DemedtsRomain A Pauwels
Apr 23, 2005·Immunity·Cheng-Ming SunRichard Lo-Man
May 6, 2005·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ken-Ichi TakanoNorimasa Sawada
Citations
Apr 12, 2007·Viral Immunology·Sasha J FachRandy E Sacco
Feb 19, 2009·Immunology and Cell Biology·Shanjana AwasthiGary White
Nov 10, 2010·PloS One·Stéphanie Ferret-BernardFabrice Laurent
Feb 15, 2011·Veterinary Research·Stéphanie Ferret-BernardFabrice Laurent
Dec 4, 2012·Viruses·Rachel J Derscheid, Mark R Ackermann
Dec 26, 2008·Veterinary Parasitology·M Yavuz GulbaharY Emre Beyhan
Aug 23, 2008·Journal of Immunological Methods·Eliane Foulon, Gilles Foucras
Jan 29, 2008·Developmental and Comparative Immunology·Caroline Piercey AkessonMona Aleksandersen
Aug 10, 2007·Veterinary Immunology and Immunopathology·Liying Lei, Jesse M Hostetter
Jun 17, 2009·Parasite Immunology·T N McNeillyJ B Matthews
Nov 12, 2014·Annual Review of Animal Biosciences·Artur SummerfieldMeret Ricklin
Sep 22, 2016·Mucosal Immunology·N TorowE S Gollwitzer
Mar 27, 2013·Veterinary Pathology·R J Derscheid, M R Ackermann
Dec 29, 2007·Immunological Investigations·W R Waters, R E Sacco
Dec 1, 2017·Frontiers in Immunology·Laura Lambert, Fiona J Culley
Mar 14, 2008·Clinical and Vaccine Immunology : CVI·Liying LeiJesse M Hostetter
Mar 16, 2019·Parasites & Vectors·QiangQiang WangXiangRui Li