Abstract
The amine- and peptide-producing pulmonary neuroendocrine cells (PNEC) are widely distributed within the airway mucosa of mammalian lung as solitary cells and innervated clusters, neuroepithelial bodies (NEB), which function as airway O2 sensors. These cells express Cftr and hence could play a role in the pathophysiology of cystic fibrosis (CF) lung disease. We performed confocal microscopy and morphometric analysis on lung sections from Cftr-/- (null), Cftr+/+, and Cftr+/- (control) mice at developmental stages E20, P5, P9, and P30 to determine the distribution, frequency, and innervation of PNEC/NEB, innervation and cell mass of airway smooth muscle, and neuromuscular junctions using synaptic vesicle protein 2, smooth muscle actin, and synaptophysin markers, respectively. The mean number of PNEC/NEB in Cftr-/- mice was significantly reduced compared with control mice at E20, whereas comparable or increased numbers were observed postnatally. NEB cells in Cftr null mice showed a significant reduction in intracorpuscular nerve endings compared with control mice, which is consistent with an intrinsic abnormality of the PNEC system. The airways of Cftr-/- mice showed reduced density (approximately 20-30%) of smooth muscle innervat...Continue Reading
References
May 22, 1992·The Journal of Comparative Neurology·P BalukD M McDonald
Jan 1, 1988·The American Review of Respiratory Disease·D E JohnsonB G Landrum
Oct 1, 1995·The Journal of Clinical Investigation·B G ZeiherK R Thomas
Oct 1, 1994·Physiological Reviews·M Pusch, T J Jentsch
Sep 9, 1993·Nature·C YoungsonE Cutz
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·D WangE Cutz
Dec 20, 1993·Annals of the New York Academy of Sciences·T J JentschK Steinmeyer
Jul 14, 1997·Journal of the Autonomic Nervous System·A Van Lommel, J M Lauweryns
Jan 31, 1998·The Journal of Clinical Investigation·G KentA K Tanswell
Dec 22, 1998·Pediatric Pulmonology·H ScherP B McCray
Jan 29, 1999·Physiological Reviews·J M Pilewski, R A Frizzell
May 1, 1999·Archives of Histology and Cytology·A Van LommelJ M Lauweryns
Jun 29, 1999·Respiration Physiology·E Cutz, A Jackson
Sep 14, 1999·The American Journal of Physiology·R T WeylerA E Mulberg
Jun 6, 2000·Nature·L V BorovikovaK J Tracey
May 15, 2001·American Journal of Respiratory Cell and Molecular Biology·M J MhannaM A Haxhiu
Jun 21, 2001·American Journal of Respiratory Cell and Molecular Biology·K U HongB R Stripp
Aug 16, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·H YegerE Cutz
Mar 8, 2002·The Journal of Physiology·X W FuE Cutz
Mar 29, 2002·American Journal of Respiratory Cell and Molecular Biology·Jenny TolletMalcolm P Sparrow
Oct 25, 2002·American Journal of Respiratory Cell and Molecular Biology·Jie PanHerman Yeger
Dec 21, 2002·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Dirk AdriaensenJean-Pierre Timmermans
Aug 6, 2003·The Journal of Experimental Medicine·Christina NassensteinJohann Christian Virchow
Oct 1, 2003·Respiratory Physiology & Neurobiology·Malcolm P Sparrow, Jasmine P Lamb
Jan 1, 2004·Progress in Brain Research·Christina NassensteinArmin Braun
Feb 7, 2004·Pediatric Research·Monique BonoraMarie Christiane Brahimi-Horn
Feb 18, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Jie PanErnest Cutz
Jul 29, 2004·BMC Genetics·J Craig CohenJanet E Larson
May 13, 2005·The New England Journal of Medicine·Steven M RoweEric J Sorscher
Citations
Sep 25, 2009·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·William D Travis
Jun 17, 2011·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Martina WilkeBob J Scholte
Jan 31, 2009·Molecular Therapy : the Journal of the American Society of Gene Therapy·Daniel J Weiss
Mar 15, 2008·The Journal of Biological Chemistry·Enid R NeptuneHarry C Dietz
Jun 16, 2012·Journal of Pediatric Gastroenterology and Nutrition·Robert C De LisleRacquel Mueller
Jul 14, 2010·American Journal of Respiratory and Critical Care Medicine·David K MeyerholzMichael J Welsh
Feb 2, 2008·American Journal of Respiratory Cell and Molecular Biology·Tom N HilliardEric W F W Alton
Jul 16, 2008·Proceedings of the American Thoracic Society·Daniel J WeissDarwin J Prockop
Jun 10, 2011·Proceedings of the American Thoracic Society·Daniel J WeissDarwin J Prockop
Oct 31, 2013·American Journal of Respiratory and Critical Care Medicine·Ryan J AdamDavid A Stoltz
Nov 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Elizabeth A HinesXin Sun
Jan 9, 2014·Physiological Reports·Helen L WallaceTheodor Burdyga
Sep 3, 2008·Histochemistry and Cell Biology·Inge BrounsDirk Adriaensen
Dec 17, 2008·Experimental Lung Research·Helen L WallaceKevin W Southern
Aug 8, 2014·Frontiers in Pediatrics·Mary E Sunday
Nov 20, 2014·PloS One·Jie PanHerman Yeger
May 3, 2008·The Journal of Physiology·Elise BonvinMonique Bonora
Nov 16, 2007·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Ernest CutzJie Pan
Apr 4, 2018·Respiratory Research·Alexandra McCarronDavid Parsons
Jun 3, 2010·Annals of Neurology·Anne-Marie LamhonwahIngrid Tein
Mar 17, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Robert C De Lisle
Aug 8, 2018·Respiratory Research·Kalina R Atanasova, Leah R Reznikov
Jun 24, 2009·Jornal brasileiro de pneumologia : publicaça̋o oficial da Sociedade Brasileira de Pneumologia e Tisilogia·Ester Nei Aparecida Martins ColettaCarlos Alberto de Castro Pereira
Jul 20, 2018·Nature Communications·Wenguang YinDidier Y R Stainier
Jul 1, 2020·Journal of Cystic Fibrosis : Official Journal of the European Cystic Fibrosis Society·Anna SemaniakouValerie Chappe
Feb 2, 2021·Pediatric Pulmonology·Bin WangGiovanni A Rossi
Oct 28, 2020·Developmental Cell·Jinhao XuXin Sun