PMID: 11325613Apr 28, 2001Paper

Pulmonary surfactant phosphatidylcholine transport bypasses the brefeldin A sensitive compartment of alveolar type II cells

Biochimica Et Biophysica Acta
K OsanaiD R Voelker

Abstract

Brefeldin A (BFA) causes disassembly of the Golgi apparatus and blocks protein transport to this organelle from the endoplasmic reticulum. However, there still remains considerable ambiguity regarding the involvement of the Golgi apparatus in glycerolipid transport pathways. We examined the effects of BFA upon the intracellular translocation of phosphatidylcholine in alveolar type II cells, that synthesize, transport, store and secrete large amounts of phospholipid for regulated exocytosis. BFA at concentrations as high as 10 microg/ml failed to alter the assembly of phosphatidylcholine into lamellar bodies, the specialized storage organelles for pulmonary surfactant. The same concentration of BFA was also ineffective at altering the secretion of newly synthesized phosphatidylcholine from alveolar type II cells. In contrast, concentrations of the drug of 2.5 microg/ml completely arrested newly synthesized lysozyme secretion from the same cells, indicating that BFA readily blocked protein transport processes in alveolar type II cells. The disassembly of the Golgi apparatus in alveolar type II cells following BFA treatment was also demonstrated by showing the redistribution of the resident Golgi protein MG-160 to the endoplasmic ...Continue Reading

References

Apr 1, 1992·The American Journal of Physiology·J J Batenburg
Mar 1, 1992·The Journal of Cell Biology·R D KlausnerJ Lippincott-Schwartz
Jul 1, 1991·American Journal of Respiratory Cell and Molecular Biology·A SuwabeD R Voelker
Jan 1, 1991·Annual Review of Biochemistry·K W Wirtz
Mar 12, 1991·Biochimica Et Biophysica Acta·M Koval, R E Pagano
Jul 1, 1990·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·S CroulN K Gonatas
Nov 1, 1988·The American Review of Respiratory Disease·G SinghR J Mason
Aug 1, 1985·The Journal of Cell Biology·M R Kaplan, R D Simoni
Aug 1, 1985·The Journal of Cell Biology·M R Kaplan, R D Simoni
Aug 29, 1983·Biochimica Et Biophysica Acta·A ChanderA B Fisher
Aug 4, 1994·Biochimica Et Biophysica Acta·P J Trotter, D R Voelker
Mar 29, 1994·Proceedings of the National Academy of Sciences of the United States of America·D E WarnockJ U Baenziger
Mar 15, 1996·Science·R Schekman, L Orci
Apr 12, 1996·Science·J E Rothman, F T Wieland
Nov 12, 1996·Proceedings of the National Academy of Sciences of the United States of America·N MorinagaM Vaughan
Dec 16, 1997·Proceedings of the National Academy of Sciences of the United States of America·N MorinagaM Vaughan
Jun 13, 1998·Progress in Lipid Research·G Daum, J E Vance
Jul 11, 1998·Molecular Biology of the Cell·J F PresleyJ Lippincott-Schwartz
Jul 29, 1998·Histochemistry and Cell Biology·A Dinter, E G Berger
Nov 14, 1998·Biochimica Et Biophysica Acta·R J Mason, D R Voelker
Dec 9, 1998·American Journal of Respiratory Cell and Molecular Biology·K OsanaiD R Voelker
Apr 27, 1999·Biochimica Et Biophysica Acta·L Liscum, N J Munn
Mar 18, 2000·The Journal of Biological Chemistry·A KorimilliS H Guttentag
Jun 17, 2000·Biochimica Et Biophysica Acta·D R Voelker
Jun 17, 2000·Biochimica Et Biophysica Acta·G van Meer, J C Holthuis
Jun 17, 2000·Biochimica Et Biophysica Acta·E J Blanchette-Mackie
Jul 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·S HeinoE Ikonen
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

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Citations

Sep 24, 2002·Seminars in Cell & Developmental Biology·Timothy E WeaverMildred Stahlman
Jan 21, 2006·Respirology : Official Journal of the Asian Pacific Society of Respirology·Kazuhiro OsanaiHirohisa Toga
Oct 3, 2012·Biochimica Et Biophysica Acta·Marianna Agassandian, Rama K Mallampalli
Dec 4, 2012·Biochimica Et Biophysica Acta·Victoria GossAnthony D Postle
Oct 4, 2008·The American Journal of Pathology·Kazuhiro OsanaiDennis R Voelker
Oct 30, 2016·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Bárbara OlmedaJesus Pérez-Gil
Apr 22, 2005·Biological Chemistry·Kazuhiro OsanaiDennis R Voelker
May 15, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Alexandra V AndreevaTatyana A Voyno-Yasenetskaya
Jun 17, 2010·Physiology·Jesús Perez-Gil, Timothy E Weaver
Nov 10, 2009·American Journal of Physiology. Lung Cellular and Molecular Physiology·Kazuhiro OsanaiHirohisa Toga
Jun 20, 2020·Cell and Tissue Research·Kazuhiro OsanaiKeiji Takahashi
Aug 1, 2018·International Journal of Molecular Sciences·Kazuhiro Osanai

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