Pulmonary surfactant protein SP-C counteracts the deleterious effects of cholesterol on the activity of surfactant films under physiologically relevant compression-expansion dynamics.

Biophysical Journal
L Gómez-GilJesús Pérez-Gil

Abstract

The presence of cholesterol is critical in defining a dynamic lateral structure in pulmonary surfactant membranes. However, an excess of cholesterol has been associated with impaired surface activity of surfactant. It has also been reported that surfactant protein SP-C interacts with cholesterol in lipid/protein interfacial films. In this study, we analyzed the effect of SP-C on the thermodynamic properties of phospholipid membranes containing cholesterol, and the ability of lipid/protein complexes containing cholesterol to form and respread interfacial films capable of producing very low surface tensions upon repetitive compression-expansion cycling. SP-C modulates the effect of cholesterol to reduce the enthalpy associated with the gel-to-liquid-crystalline melting transition in dipalmitoylphosphatidylcholine (DPPC) bilayers, as analyzed by differential scanning calorimetry. The presence of SP-C affects more subtly the effects of cholesterol on the thermotropic properties of ternary membranes, mimicking more closely the lipid composition of native surfactant, where SP-C facilitates the miscibility of the sterol. Incorporation of 1% or 2% SP-C (protein/phospholipid by weight) promotes almost instantaneous adsorption of suspens...Continue Reading

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Citations

Sep 22, 2012·European Biophysics Journal : EBJ·Bárbara OlmedaJesús Pérez-Gil
Jun 19, 2010·The Journal of Biological Chemistry·Andrea RavasioJesús Pérez-Gil
Nov 13, 2015·Colloids and Surfaces. B, Biointerfaces·Eduardo GuzmánFrancesca Ravera
Sep 6, 2011·Biophysical Journal·Nathan J BrownAnnelise E Barron
Sep 29, 2011·Microbes and Infection·Jennifer R Glasser, Rama K Mallampalli
Feb 15, 2014·Biochimica Et Biophysica Acta·Elena Lopez-Rodriguez, Jesús Pérez-Gil
Aug 9, 2011·Biochimica Et Biophysica Acta·Clara Aicart-RamosIgnacio Rodriguez-Crespo
Feb 2, 2011·Biophysical Journal·Elena Lopez-RodriguezJesus Perez-Gil
Oct 21, 2016·Biophysical Journal·Nuria RoldanBegoña García-Álvarez
Oct 30, 2016·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Bárbara OlmedaJesus Pérez-Gil
Jul 15, 2017·Therapeutic Hypothermia and Temperature Management·Chiara AutilioDaniele De Luca
Sep 3, 2010·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Christopher J PynnWolfgang Bernhard
Jan 11, 2020·American Journal of Respiratory Cell and Molecular Biology·Jannik RuwischElena Lopez-Rodriguez
May 14, 2020·American Journal of Respiratory Cell and Molecular Biology·Elena Lopez-Rodriguez, Matthias Ochs
Jun 21, 2014·Physical Chemistry Chemical Physics : PCCP·F T HaneZ Leonenko
May 30, 2020·International Journal of Molecular Sciences·Olga CañadasJesús Pérez-Gil
Mar 24, 2021·Archives of Biochemistry and Biophysics·José Carlos Castillo-SánchezJesús Pérez-Gil
Aug 5, 2021·Current Research in Toxicology·Emilie Da SilvaJorid B Sørli
Apr 26, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Dunja LukovicJesus Pérez-Gil
Nov 3, 2020·Biochimica Et Biophysica Acta. Biomembranes·Emilie Da SilvaJorid Birkelund Sørli

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