PMID: 19943231Nov 28, 2009Paper

Pulmonary tissue engineering using dual-compartment polymer scaffolds with integrated vascular tree

The International Journal of Artificial Organs
Clemens S FritscheJ P Vacanti

Abstract

The persistent shortage of donor organs for lung transplantation illustrates the need for new strategies in organ replacement therapy. Pulmonary tissue engineering aims at developing viable hybrid tissue for patients with chronic respiratory failure. Dual-chamber polymer constructs that mimic the characteristics of the pulmonary air-blood interface were fabricated by microfabrication techniques using the biocompatible polymer polydimethylsiloxane. One compartment ("vascular chamber") was designed as a capillary network to mimic the pulmonary microvasculature. The other compartment ("parenchymal chamber") was designed to permit gas exchange. Immortalized mouse lung epithelium cells (MLE-12) were cultured on the surface of polystyrene microcarrier beads. These beads were subsequently injected into the parenchymal chamber of the dual-chamber microsystems. The vascular compartment was perfused with cell culture medium in a bioreactor and the construct was maintained in culture for 1 week. The microcarriers evenly distributed MLE-12 cells on the parenchymal compartment surface. Confluent cell layers were confirmed by fluorescent and electron microscopy. Adequate proliferation of MLE-12 cells within the construct was monitored via th...Continue Reading

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Citations

Dec 29, 2009·The International Journal of Artificial Organs·Gerardo Catapano, Jon B Klein
Aug 6, 2014·Nature Biotechnology·Sangeeta N Bhatia, Donald E Ingber
Jan 7, 2017·Circulation Research·Anton V BorovjaginJianyi Zhang
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