Pulp stem cells with hepatocyte growth factor overexpression exhibit dual effects in rheumatoid arthritis.

Stem Cell Research & Therapy
Xiwen DongHua Wang

Abstract

To investigate the therapeutic effect of human dental pulp stem cells (DPSCs) transfected with adenovirus expressing hepatocyte growth factor (HGF) in a mouse model of collagen-induced arthritis (CIA). DPSCs were modified with Ad-HGF to produce HGF-overexpressing DPSCs, DPSCs-HGF. In experimental mouse CIA model, DPSCs-HGF and DPSCs-Null (modified with Ad-Null) were engrafted via intravenously after disease onset, which was determined by the presence of joint swelling. The therapeutic effects on joints were evaluated at 49 days after collagen injection by histopathological analysis and microcomputed tomography imaging. The inflammatory cytokines were analyzed both in sera and joints via MILLIPLEX kit and immunohistochemical staining, respectively, and the regulatory T cells (Tregs) were analyzed in peripheral blood by using flow cytometry. Furthermore, primary fibroblast-like synoviocytes were isolated, colony formation analysis and FACS were performed to evaluate the effect of HGF on the proliferation and cell cycle of FLSs. Western blot assay was carried out to clarify the signal pathway of HGF-cMet. We found that without HGF modification, DPSC transfusion was helpful in controlling autoimmune status, local synovitis, and bon...Continue Reading

References

Sep 4, 2003·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Fred R HirschWilbur A Franklin
Jun 5, 2007·Nature Protocols·David D BrandEdward F Rosloniec
Oct 4, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Katsuhide OkunishiKazuhiko Yamamoto
Nov 3, 2011·Arthritis·Misato Hashizume, Masahiko Mihara
Oct 22, 2014·SpringerPlus·Seiji ShibasakiTsuyoshi Iwasaki
Dec 6, 2014·Autoimmunity Reviews·Nicolas MolnarfiPatrice H Lalive
Jul 15, 2015·Annals of the New York Academy of Sciences·Antonio Uccelli, Nicole Kerlero de Rosbo
Dec 15, 2015·Nature Reviews. Rheumatology·Iain B McInnesJohn D Isaacs
Oct 30, 2016·Lancet·Josef S SmolenIain B McInnes
Dec 13, 2016·Current Opinion in Rheumatology·Sharon AnsboroCosimo De Bari
Jan 13, 2018·Scientific Reports·Zhuan FengPing Zhu
Jun 27, 2018·Cell Biochemistry and Function·Wenzhong QueShanghua Xu
Mar 27, 2019·Cellular & Molecular Immunology·Xiwen DongPing Zhu

❮ Previous
Next ❯

Methods Mentioned

BETA
protein assay
immunoprecipitation
enzyme-linked immunosorbent assay
ELISA
flow cytometry

Software Mentioned

CaseViewer
MAGPI Analyst
Inveon Acquisition Workplace
COBRA
Inveon Research Workplace

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.