Pulsatile atheroprone shear stress affects the expression of transient receptor potential channels in human endothelial cells

Hypertension
Florian ThiloMartin Tepel

Abstract

The goal of the study was to assess whether pulsatile atheroprone shear stress modulates the expression of transient receptor potential (TRP) channels, TRPC3, TRPC6, TRPM7, and TRPV1 mRNA, in human umbilical vascular endothelial cells. Exposure of cultured vascular endothelial cells to defined shear stress, producing a constant laminar flow (generating a shear stress of 6 dyne/cm(2)), laminar pulsatile atheroprotective flow (with a mean shear stress of 20 dyne/cm(2)), or laminar atheroprone bidirectional flow (with a mean shear stress of 0 dyne/cm(2)) differentially induced TRPC6 and TRPV1 mRNA as measured by quantitative real-time RT-PCR and normalized to GAPDH expression. Thereby, TRPC6 and TRPV1 mRNA expressions were significantly increased after 24 hours of exposure to an atheroprone flow profile compared with an atheroprotective flow profile. Furthermore, the expression of transcription factors GATA1 and GATA4 was significantly correlated with the expression of TRPC6 mRNA. In contrast, after 24 hours of constant laminar flow, the expression of TRPC6 and TRPV1 mRNA was unchanged, whereas the expression of TRPC3 and TRPM7 was significantly higher in endothelial cells exposed to shear stress in comparison with endothelial cel...Continue Reading

References

Sep 1, 1986·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·C L IvesL V McIntire
Jan 1, 1993·Journal of Cardiovascular Pharmacology·B L Langille
Dec 21, 2002·Molecular Immunology·Mauro BongrazioAndreas Zakrzewicz
Oct 11, 2003·American Journal of Physiology. Heart and Circulatory Physiology·T M PocockD O Bates
Jul 3, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Takaaki SokabeJoji Ando
Oct 7, 2004·Proceedings of the National Academy of Sciences of the United States of America·Guohao DaiMichael A Gimbrone
Jul 22, 2006·Circulation Research·Ryuji InoueYushi Ito
Nov 14, 2006·The Journal of Clinical Investigation·Koichiro KuwaharaEric N Olson
Jan 24, 2007·Physiological Reviews·Bernd NiliusJohn A Peters
Jan 30, 2007·FEBS Letters·Sven ChlenchAndreas Zakrzewicz
Sep 13, 2008·Arteriosclerosis, Thrombosis, and Vascular Biology·Kathryn Smedlund, Guillermo Vazquez
Nov 26, 2008·Nature Clinical Practice. Cardiovascular Medicine·Peter F Davies
Feb 7, 2009·Nature Reviews. Molecular Cell Biology·Cornelia Hahn, Martin A Schwartz
Jun 27, 2009·The Journal of Biological Chemistry·Chun FanQing Kenneth Wang
Nov 17, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Daniel E ConwayLarry V McIntire
Jul 29, 2010·Journal of Cellular Physiology·Margret HohbergAndreas Zakrzewicz
Jul 31, 2010·Journal of Biomechanical Engineering·Leonie RouleauRichard L Leask
Dec 15, 2010·Circulation Journal : Official Journal of the Japanese Circulation Society·Alvaro YogiRhian M Touyz
Apr 30, 2011·Arteriosclerosis, Thrombosis, and Vascular Biology·Bradley D GelfandBrett R Blackman
Sep 13, 2011·Cardiovascular Research·Liqun MaMartin Tepel
Sep 13, 2011·PloS One·Cornelia HahnMartin Alexander Schwartz
Oct 19, 2011·Antioxidants & Redox Signaling·Florian ThiloMartin Tepel

❮ Previous
Next ❯

Citations

Jan 1, 2013·Current Opinion in Nephrology and Hypertension·Michael Hollis, Donna H Wang
Feb 21, 2016·Pharmacology & Therapeutics·Susumu OhyaKatsuhiko Muraki
Oct 18, 2015·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·R WeiY Huo
Dec 21, 2012·Acta Physiologica·M KassmannM Gollasch
Sep 11, 2016·The Journal of Physiological Sciences : JPS·Kayoko OdaYoshihiro Ishikawa
Dec 5, 2014·American Journal of Physiology. Cell Physiology·Zhao ZengZhi-Gang Xiong
Dec 23, 2020·International Journal of Molecular Sciences·Giulia GagnoAneta Aleksova
Sep 28, 2021·Frontiers in Cell and Developmental Biology·Laura Locatelli, Jeanette A M Maier

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atherosclerosis Disease Progression

Atherosclerosis is the buildup of plaque on artery walls, causing stenosis which can eventually lead to clinically apparent cardiovascular disease. Find the latest research on atherosclerosis disease progression here.