PMID: 19912809Aug 1, 1991Paper

Pulsatile GnRH stimulation increases steady-state mRNA levels for FSHbeta, LHbeta, and alpha subunits in superfused pituitary cell cultures

Molecular and Cellular Neurosciences
A JakubowiakA Steinberger

Abstract

The pituitary secretion of FSH and LH is controlled by pulsatile GnRH stimulation of specific magnitude and frequency, and changes in the mode of stimulation can dramatically alter the pattern of gonadotropin secretion. We explored the effects of different GnRH treatments on the secretion of gonadotropins and levels of mRNA for the gonadotropin subunits in superfused cultures of immature rat pituitary cells. After 4 days of stationary culture in the absence of GnRH, the cells were exposed during superfusion to different GnRH treatments for up to 20 h. Each GnRH pulse (6 min/h, 10 nM) elicited pulsatile release of both gonadotropins, causing a 2- to 3-fold increase of mean hourly secretion of FSH and 5- to 6-fold increase of LH. The magnitude of gonadotropin responses after 4, 10, and 20 h of stimulation with GnRH pulses was not significantly different. In contrast, mRNA levels for different subunits varied depending on the duration of GnRH exposure. Only FSHbeta mRNA was increased (2.5 +/-0.4-fold,P < 0.05) after 4 h of stimulation, and only after 10 h was the a subunit mRNA also significantly increased (2.1 +/- 0.3-fold, P < 0.05). After 20 h of GnRH exposure, both mRNA levels became further elevated (4-to 7-fold vs no GnRH) a...Continue Reading

References

Feb 1, 1990·Endocrine Reviews·S D GharibW W Chin
Oct 1, 1990·Molecular and Cellular Endocrinology·J E Mercer

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