Pulsatile Hyperglycaemia Induces Vascular Oxidative Stress and GLUT 1 Expression More Potently than Sustained Hyperglycaemia in Rats on High Fat Diet

PloS One
Günaj RakipovskiKirsten Raun

Abstract

Pulsatile hyperglycaemia resulting in oxidative stress may play an important role in the development of macrovascular complications. We investigated the effects of sustained vs. pulsatile hyperglycaemia in insulin resistant rats on markers of oxidative stress, enzyme expression and glucose metabolism in liver and aorta. We hypothesized that liver's ability to regulate the glucose homeostasis under varying states of hyperglycaemia may indirectly affect oxidative stress status in aorta despite the amount of glucose challenged with. Animals were infused with sustained high (SHG), low (SLG), pulsatile (PLG) glucose or saline (VEH) for 96 h. Oxidative stress status and key regulators of glucose metabolism in liver and aorta were investigated. Similar response in plasma lipid oxidation was observed in PLG as in SHG. Likewise, in aorta, PLG and SHG displayed increased expression of glucose transporter 1 (GLUT1), gp-91PHOX and super oxide dismutase (SOD), while only the PLG group showed increased accumulation of oxidative stress and oxidised low density lipoprotein (oxLDL) in aorta. Pulsatile hyperglycaemia induced relatively higher levels of oxidative stress systemically and in aorta in particular than overt sustained hyperglycaemia t...Continue Reading

References

Feb 1, 1995·Metabolism: Clinical and Experimental·F Assimacopoulos-JeannetB Jeanrenaud
Aug 1, 1996·Annals of Medicine·M R TaskinenM Syvänne
Mar 11, 2000·American Journal of Physiology. Endocrinology and Metabolism·M NawanoT Asano
Jun 16, 2000·Diabetologia·D A SlatterA J Bailey
Oct 12, 2001·American Journal of Physiology. Endocrinology and Metabolism·A RissoA Ceriello
Mar 5, 2003·Journal of Biochemical and Molecular Toxicology·A C MaritimJ B Watkins
Jun 14, 2003·Journal of the Renin-angiotensin-aldosterone System : JRAAS·David G HarrisonKathy K Griendling
Sep 12, 2003·Trends in Pharmacological Sciences·Hua CaiDavid G Harrison
Jan 27, 2004·Endothelium : Journal of Endothelial Cell Research·Robert W O'DonnellJames A Holland
Jul 17, 2004·Histochemistry and Cell Biology·George L King, Mary R Loeken
Sep 7, 2004·Diabetologia·B G ToppD T Finegood
Sep 16, 2005·Liver International : Official Journal of the International Association for the Study of the Liver·Rie SugimotoMakoto Nakamuta
Oct 20, 2005·American Journal of Physiology. Endocrinology and Metabolism·Edward W KraegenNeil B Ruderman
Mar 1, 2006·Diabetes/metabolism Research and Reviews·Eugenio Cersosimo, Ralph A DeFronzo
Apr 13, 2006·JAMA : the Journal of the American Medical Association·Louis MonnierClaude Colette
Apr 13, 2006·JAMA : the Journal of the American Medical Association·Michael Brownlee, Irl B Hirsch
Aug 5, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Kosuke AzumaHirotaka Watada
Aug 8, 2006·Nutrition, Metabolism, and Cardiovascular Diseases : NMCD·Lisa QuagliaroAntonio Ceriello
Aug 11, 2006·Obesity·Fermín I MilagroJ Alfredo Martínez
Mar 6, 2007·Clinica Chimica Acta; International Journal of Clinical Chemistry·Jens Lykkesfeldt
Mar 16, 2007·Proceedings of the National Academy of Sciences of the United States of America·Giovanni G CamiciFrancesco Cosentino
Jul 20, 2007·European Journal of Clinical Investigation·M ArtwohlS M Baumgartner-Parzer
Aug 28, 2007·The American Journal of Cardiology·James H O'Keefe, David S H Bell
Feb 6, 2008·The Veterinary Clinics of North America. Small Animal Practice·Ling GaoHua Cai
Jul 25, 2008·The Biochemical Journal·Loranne Agius
Sep 17, 2008·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Yan ZhaoNagara Tamaki
May 1, 2009·Cardiovascular Diabetology·Koichi Node, Teruo Inoue
Oct 7, 2009·Proceedings of the National Academy of Sciences of the United States of America·Kyle L HoehnDavid E James
Dec 17, 2010·Trends in Pharmacological Sciences·Tony Tiganis
Jan 22, 2011·British Journal of Pharmacology·Cristina M SenaRaquel M Seiça
May 31, 2011·Biochimica Et Biophysica Acta·Jolita CiapaiteJeanine J Prompers
Sep 22, 2011·Diabetes & Vascular Disease Research : Official Journal of the International Society of Diabetes and Vascular Disease·Günaj RakipovskiJens Lykkesfeldt

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