Pulsatilla decoction inhibits Candida albicans proliferation and adhesion in a mouse model of vulvovaginal candidiasis via the Dectin-1 signaling pathway

Journal of Ethnopharmacology
Lilin YangSongping Luo

Abstract

Pulsatilla decoction (PD) is a classical prescription in Traditional Chinese Medicine (TCM) and has been reported to have inhibitory effects on Candida albicans proliferation. To investigate the therapeutic effects of PD in the treatment of Vulvovaginal candidiasis (VVC) and elucidate the potential mechanism. Female BALB/c mice (N = 90) were randomized to six treatment groups, including the Control group, Model group, three PD groups and Fluconazole group which served as a positive control (20 mg/kg weekly). The three PD groups (low dose group, medium dose group and high dose group) were given a daily intragastric gavage of PD at doses of 5, 10 and 20 g/kg, respectively. Five animals from each group were euthanized on Day 4, Day 7 and Day 14 after treatment. Colony forming unit (CFU) was measured by the serial dilution method. The degree of infection was assessed by Gram staining, Periodic acid schiff (PAS) staining, Hematoxylin and eosin (H&E) staining and Scanning electron microscopy (SEM). The serum inflammation levels were determined by a Luminex assay. Gene and protein expression levels of components of the Dectin-1 signaling pathway were determined by Real-time PCR, Western-blot and immunohistochemistry, respectively. The...Continue Reading

Citations

Jun 11, 2020·BMC Complementary Medicine and Therapies·Shaohua HuangfuBin Jiang
Nov 30, 2018·American Journal of Rhinology & Allergy·Andy J Jin, Christopher J Chin
May 28, 2021·Cancer Biotherapy & Radiopharmaceuticals·Yanghua JieWeidong Chen
Aug 6, 2020·Current Drug Targets·Graziela Vargas Rigo, Tiana Tasca

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