PMID: 6991253Apr 1, 1980Paper

Pulse-fluorometry study on actin and heavy meromyosin using F-actin labelled with N-(1-pyrene)maleimide

European Journal of Biochemistry
T Kouyama, K Mihashi

Abstract

The single-photoelectron counting technique was used for measurement of the fluorescence decay kinetics of N-(1-pyrene)maleimide conjugated to the fast reactive cysteine of actin. The fluorescence decay curve of the labelled G-actin could not be described by a single-exponential function but by a double-exponential function. Polymerization of actin was accompanied by significant changes in the decay parameters of the two decay components. We found that the ionic strength, which plays an important role in the G-F equilibrium, scarcely affected these parameters provided that the labelled actin exists in the monomeric state. Thus it is suggested that the conformational change of actin protomer occurs at the time of association. When heavy meromyosin was added to the labelled F-actin, the decay parameter changed monotonically on increasing saturation of binding of heavy meromyosin and it levelled-off around a ratio of heavy meromyosin:actin of 0.5 mol/mol. Decay parameters under the influence of heavy meromyosin had values intermediate between those observed for the labelled G-actin and for the labelled F-actin. Therefore, it is suggested that binding of heavy meromyosin to F-actin alters the conformation of actin protomer towards ...Continue Reading

References

Jun 29, 1976·Biochemistry·C W Wu, L R Yarbrough
Jun 16, 1975·European Journal of Biochemistry·H Strzelecka-GolaszewskaW Drabikowski
May 28, 1969·Journal of Molecular Biology·S LoweyH Baker
Oct 25, 1974·Journal of Molecular Biology·F D Carlson, A B Fraser
Oct 1, 1973·Biophysical Journal·I IsenbergR Hanson
Jul 25, 1971·Biochimica Et Biophysica Acta·S Abe, K Maruyama
Nov 28, 1971·Journal of Molecular Biology·S Fujime, S Ishiwata
Aug 13, 1968·Biochimica Et Biophysica Acta·K K Tsuboi
Feb 25, 1969·Biochimica Et Biophysica Acta·K Tawada
Jan 1, 1962·Journal of Molecular Biology·F OOSAWA, M KASAI

❮ Previous
Next ❯

Citations

Nov 21, 2009·The Journal of Biological Chemistry·Ikuko FujiwaraJohn A Hammer
Jul 16, 2010·The Journal of Biological Chemistry·Adam ZwolakNico Tjandra
Jun 12, 2010·The Journal of Biological Chemistry·Adam ZwolakNico Tjandra
Jun 25, 2005·Molecular Biology of the Cell·Herwig SchülerKai Matuschewski
Dec 2, 1985·European Journal of Biochemistry·M Mossakowska, H Strzelecka-Gołaszewska
Nov 19, 2015·Plant & Cell Physiology·Saku T KijimaTaro Q P Uyeda
Sep 30, 2008·Arthritis Research & Therapy·Teresia M OsbornMaria Bokarewa
Nov 28, 2007·BMC Biology·Cathryn KabacoffDouglas N Robinson
Jun 4, 1999·Biophysical Journal·N Fuller, R P Rand
Oct 12, 2000·Biophysical Chemistry·E Kim, E Reisler
Aug 15, 1988·European Journal of Biochemistry·M MossakowskaH Strzelecka-Gołaszewska
Jan 1, 1984·CRC Critical Reviews in Biochemistry·P C Leavis, J Gergely
Mar 22, 1996·The Journal of Biological Chemistry·X YaoJ G Forte
Mar 1, 1992·The American Journal of Physiology·S BurlacuJ Borejdo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.