Pulsed Electromagnetic Field Regulates MicroRNA 21 Expression to Activate TGF-β Signaling in Human Bone Marrow Stromal Cells to Enhance Osteoblast Differentiation

Stem Cells International
Nagarajan SelvamuruganNicola C Partridge

Abstract

Pulsed electromagnetic fields (PEMFs) have been documented to promote bone fracture healing in nonunions and increase lumbar spinal fusion rates. However, the molecular mechanisms by which PEMF stimulates differentiation of human bone marrow stromal cells (hBMSCs) into osteoblasts are not well understood. In this study the PEMF effects on hBMSCs were studied by microarray analysis. PEMF stimulation of hBMSCs' cell numbers mainly affected genes of cell cycle regulation, cell structure, and growth receptors or kinase pathways. In the differentiation and mineralization stages, PEMF regulated preosteoblast gene expression and notably, the transforming growth factor-beta (TGF-β) signaling pathway and microRNA 21 (miR21) were most highly regulated. PEMF stimulated activation of Smad2 and miR21-5p expression in differentiated osteoblasts, and TGF-β signaling was essential for PEMF stimulation of alkaline phosphatase mRNA expression. Smad7, an antagonist of the TGF-β signaling pathway, was found to be miR21-5p's putative target gene and PEMF caused a decrease in Smad7 expression. Expression of Runx2 was increased by PEMF treatment and the miR21-5p inhibitor prevented the PEMF stimulation of Runx2 expression in differentiating cells. Th...Continue Reading

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Citations

Dec 21, 2017·BioMed Research International·Chen-Ying SuHsu-Wei Fang
Mar 26, 2019·Bioelectromagnetics·Carlo GalliStefano Guizzardi
Apr 23, 2020·Molecular Medicine Reports·Yuan XiongGuohui Liu
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Mar 7, 2021·International Journal of Molecular Sciences·Chiara MazziottaFernanda Martini
Jul 25, 2021·International Journal of Molecular Sciences·Laura CaliognaGianluigi Pasta

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