Pulsed Electromagnetic Fields Modulate miRNAs During Osteogenic Differentiation of Bone Mesenchymal Stem Cells: a Possible Role in the Osteogenic-angiogenic Coupling.

Stem Cell Reviews and Reports
Monica De MatteiValeria Bertagnolo

Abstract

Despite the high intrinsic ability of bone tissue to regenerate, bone healing fails in some pathological conditions and especially in the presence of large defects. Due to the strong relationship between bone development and vascularization during in vivo bone formation and repair, strategies promoting the osteogenic-angiogenic coupling are crucial for regenerative medicine. Increasing evidence shows that miRNAs play important roles in controlling osteogenesis and bone vascularization and are important tool in medical research although their clinical use still needs to optimize miRNA stability and delivery. Pulsed electromagnetic fields (PEMFs) have been successfully used to enhance bone repair and their clinical activity has been associated to their ability to promote the osteogenic differentiation of human mesenchymal stem cells (hMSCs). In this study we investigated the potential ability of PEMF exposure to modulate selected miRNAs involved in the osteogenic differentiation of human bone mesenchymal stem cells (hBMSCs). We show that, during in vitro hBMSC differentiation, PEMFs up-modulate the expression of miR-26a and miR-29b, which favor osteogenic differentiation, and decrease miR-125b which acts as an inhibitor miRNA. As...Continue Reading

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Citations

Jan 21, 2021·International Journal of Molecular Sciences·Katia VaraniMonica De Mattei
Mar 7, 2021·International Journal of Molecular Sciences·Chiara MazziottaFernanda Martini
Jul 22, 2021·Cell and Tissue Research·Gianfranco Giorgi, Brunella Del Re

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Methods Mentioned

BETA
ELISA
PCR

Software Mentioned

GraphPad
GraphPad Prism

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