Pulsed electromagnetic fields promote survival and neuronal differentiation of human BM-MSCs

Life Sciences
Enerelt UrnukhsaikhanJung-Kueg Park

Abstract

Pulsed electromagnetic fields (PEMF) are known to affect biological properties such as differentiation, regulation of transcription factor and cell proliferation. However, the cell-protective effect of PEMF exposure is largely unknown. The aim of this study is to understand the mechanisms underlying PEMF-mediated suppression of apoptosis and promotion of survival, including PEMF-induced neuronal differentiation. Treatment of induced human BM-MSCs with PEMF increased the expression of neural markers such as NF-L, NeuroD1 and Tau. Moreover, treatment of induced human BM-MSCs with PEMF greatly decreased cell death in a dose- and time-dependent manner. There is evidence that Akt and Ras are involved in neuronal survival and protection. Activation of Akt and Ras results in the regulation of survival proteins such as Bad and Bcl-xL. Thus, the Akt/Ras signaling pathway may be a desirable target for enhancing cell survival and treatment of neurological disease. Our analyses indicated that PEMF exposure dramatically increased the activity of Akt, Rsk, Creb, Erk, Bcl-xL and Bad via phosphorylation. PEMF-dependent cell protection was reversed by pretreatment with LY294002, a specific inhibitor of phosphatidylinositol 3-kinase (PI3K). Our ...Continue Reading

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Citations

Mar 31, 2019·Analytical and Bioanalytical Chemistry·Dinesh KankanamgeAjith Karunarathne
Jun 20, 2020·Acta Neurologica Scandinavica·Claire Prener MillerOlaf B Paulson
Feb 3, 2018·Journal of Cellular and Molecular Medicine·Chenxia Hu, Lanjuan Li
Oct 14, 2020·Journal of Molecular Neuroscience : MN·Princy ChoudharySangeeta Singh
Jun 18, 2021·World Journal of Stem Cells·Xi-Min HuKun Xiong

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