PMID: 7528885Jan 1, 1995Paper

Pulsed field analysis of hprt T-cell large deletions: telomeric region breakpoint spectrum

Mutation Research
M J LippertR J Albertini

Abstract

In order to determine a large deletion breakpoint spectrum, 25 independent hprt T-lymphocyte mutants with deletions extending from hprt into the telomeric or centromeric flanking chromosomal region were analyzed by pulsed field gel electrophoresis (PFGE). PFGE was used to determine deletion sizes which allowed localization of breakpoints external to hprt to specific chromosomal positions in mutants containing an intra-hprt breakpoint. A breakpoint spectrum based on 19 large deletion mutants is reported for the Xq26 chromosomal region telomeric to hprt. A potential cluster of breakpoints (4/19) was observed approximately 60 kb from hprt. In addition, maximum recoverable deletion size was at least 3.5 Mb. Three of the 25 mutants analyzed appeared to be complex deletion events.

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Citations

Jul 1, 1996·Somatic Cell and Molecular Genetics·N D Costa, J Thacker
Jan 1, 1995·Mutation Research·M J LippertJ A Nicklas
Feb 24, 2007·Toxicology and Applied Pharmacology·Catherine B KleinToby G Rossman
May 12, 2005·Environmental and Molecular Mutagenesis·Patrick O'NeillRichard Albertini
Jan 29, 2002·Environmental and Molecular Mutagenesis·M WilliamsJ A Nicklas
Aug 26, 1998·Environmental and Molecular Mutagenesis·L R FergusonK R Tindall
Sep 15, 2020·Environmental and Molecular Mutagenesis·Bala Bhaskar GollapudiRichard J Albertini
Dec 20, 2020·Mutation Research. Reviews in Mutation Research·Richard J Albertini, Debra A Kaden

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