PMID: 7528895Jan 1, 1995Paper

Pulsed-field gel electrophoresis analysis of the repair of psoralen plus UVA induced DNA photoadducts in Saccharomyces cerevisiae

Mutation Research
M Dardalhon, Dietrich Averbeck

Abstract

In the yeast Saccharomyces cerevisiae, double-strand breaks (DSB) have been observed during the DNA repair of psoralen plus UVA induced lesions. In the present paper, we analyzed this repair step in some detail using pulsed-field gel electrophoresis (CHEF) to get a better understanding of this phenomenon with regard to the type of lesions induced and the repair pathways involved. The results confirm that, during post-treatment incubation of Saccharomyces cerevisiae cells, DSB are formed. Their appearance is dose-dependent and the rate of induction is comparable in large (chromosome IV) and small (chromosome III) chromosomes. The formation of DSB is evidenced by the breakage of linear chromosomes III and IV, but also, after high doses, by the linearization of a circular form of chromosome III. The induction of DSB appears to be highly dependent on the induction of interstrand cross-links since they are clearly present after treatments with 8-MOP plus 365 nm radiation (inducing monoadducts and cross-linking in DNA), but practically absent after treatment with 8-MOP plus 405 nm radiation (inducing predominantly monoadducts) at comparable levels of photoadducts. The occurrence of DSB is dependent on the RAD2 and RAD52, but not on t...Continue Reading

References

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Citations

Aug 23, 2001·Mutation Research·M L Dronkert, R Kanaar
Sep 18, 1998·Mutation Research·M Buchwald, E Moustacchi
Mar 22, 2002·The Lancet Oncology·P J McHughJ A Hartley
Jul 18, 2009·Biochemistry·Jonathan T SczepanskiMarc M Greenberg
Feb 9, 2006·Chemical Reviews·David M NollPaul S Miller
Jul 5, 2007·FEMS Yeast Research·Michèle DardalhonDietrich Averbeck
Jul 6, 2007·The EMBO Journal·Jordan D WardSimon J Boulton
Feb 10, 2010·Environmental and Molecular Mutagenesis·Mitch McVey
Mar 3, 2010·Environmental and Molecular Mutagenesis·Karen M Vasquez
Jul 27, 2010·Environmental and Molecular Mutagenesis·The Vinh Ho, Orlando D Schärer
Jul 27, 2010·Environmental and Molecular Mutagenesis·John M Hinz
Mar 1, 1997·Critical Reviews in Toxicology·S VamvakasW K Lutz
Feb 14, 2002·Journal of Radiation Research·M BalaH C Goel
Apr 13, 2005·Biochemistry. Biokhimii︠a︡·N P Sharova
May 20, 2017·International Journal of Molecular Sciences·Lisa N ChesnerColin Campbell
Oct 15, 2005·The Journal of Biological Chemistry·Nianxiang ZhangRandy J Legerski
Nov 1, 1996·The Journal of Biological Chemistry·P CalsouB Salles
Jun 8, 2001·The Journal of Biological Chemistry·R B GreenbergW A Saffran
Jun 24, 2004·The Journal of Biological Chemistry·Wilma A SaffranJohn E Hearst
Jul 30, 1999·Journal of Medicinal Chemistry·A ChilinF Bordin
Dec 1, 1996·Molecular and Cellular Biology·A F FaruqiP M Glazer
Oct 29, 1997·Molecular and Cellular Biology·D J SegalD Carroll

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