Pulsed heat shocks enhance procollagen type I and procollagen type III expression in human dermal fibroblasts

Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)
S D DamsF P T Baaijens

Abstract

The formation of wrinkles is associated with degeneration of the collagen matrix. For regeneration of the matrix, fibroblasts need to be stimulated in producing new collagen. In this study, the effect of short-pulsed heat shocks on gene expression of procollagen type I, procollagen type III, heat shock protein (hsp)27, hsp47 and hsp70 and on the expression of remodeling markers, procollagen type I carboxy-terminal peptide (P1P) and carboxy-terminal telopeptide of type I (ICTP), of human dermal fibroblasts in vitro, is investigated. Temperatures of 45 degrees C and 60 degrees C were used for the heat shocks. The proliferation rates, viability and metabolic activity were measured directly after the pulsed heat shocks and quantitative PCR was performed at five different time points after the heat shocks. Enzyme Immuno Assays were performed to determine the concentrations of P1P and ICTP. A decreased proliferation rate of the 60 degrees C heat shocked cells was shown, whereas the viability and metabolic activity did not differ. Furthermore, gene expressions were upregulated in both 45 degrees C and 60 degrees C heat-shocked cells. However, remodeling marker analyses showed a larger amount of collagen produced by 60 degrees C heat-s...Continue Reading

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Citations

Mar 16, 2013·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Jeong Eun KimSung Eun Chang
Nov 19, 2011·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Jeong Eun KimMi Woo Lee
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Jan 22, 2011·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·S D DamsF P T Baaijens
Jul 9, 2013·Lasers in Surgery and Medicine·Rickard LiljemalmHans von Holst
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Oct 20, 2020·Electromagnetic Biology and Medicine·María Ángeles TrilloAlejandro Úbeda
Dec 29, 2020·Journal of Cosmetic Dermatology·E Beltrán-FrutosL M Pastor

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