Pulsed laser triggered high speed microfluidic fluorescence activated cell sorter.

Lab on a Chip
Ting-Hsiang WuPei-Yu Chiou

Abstract

We report a high speed and high purity pulsed laser triggered fluorescence activated cell sorter (PLACS) with a sorting throughput up to 20,000 mammalian cells s(-1) with 37% sorting purity, 90% cell viability in enrichment mode, and >90% purity in high purity mode at 1500 cells s(-1) or 3000 beads s(-1). Fast switching (30 μs) and a small perturbation volume (~90 pL) is achieved by a unique sorting mechanism in which explosive vapor bubbles are generated using focused laser pulses in a single layer microfluidic PDMS channel.

References

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Citations

Feb 23, 2013·Advanced Drug Delivery Reviews·Xin Ting ZhengChang Ming Li
Mar 29, 2012·Lab on a Chip·Yue ChenPei-Yu Chiou
Aug 7, 2012·Lab on a Chip·Wuzhou SongDemetri Psaltis
Jul 21, 2012·Lab on a Chip·Olav Gaute HellesøBalpreet Singh Ahluwalia
Sep 21, 2012·Lab on a Chip·Xiaoyun DingTony Jun Huang
Jun 20, 2013·The Analyst·Yan GaoDimitri Pappas
Apr 24, 2015·Scientific Reports·Khashayar KhoshmaneshKourosh Kalantar-zadeh
Nov 23, 2012·Electrophoresis·Yongxin SongDongqing Li
Nov 28, 2013·Biotechnology Advances·Sara BaratchiArnan Mitchell
Oct 9, 2014·The Analyst·Evgenia Yu Basova, Frantisek Foret
Jul 8, 2017·Lab on a Chip·Shinya SakumaFumihito Arai
Jan 19, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Jingjing Zhao, Zheng You
Apr 18, 2018·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Chengxun Liu
Apr 23, 2014·Nature Communications·Guillaume LajoinieMichel Versluis
May 10, 2016·Journal of Biomedical Optics·Atsuhiro IshiiMitsuhiro Terakawa
Sep 23, 2018·Electrophoresis·Yanli GongHai Jiang
May 12, 2019·Micromachines·Iordania ConstantinouMichael Knop
Jul 25, 2019·Lab on a Chip·Takanori IinoYoichiroh Hosokawa
Nov 14, 2019·Lab on a Chip·Tianlong ZhangMing Li
Sep 29, 2019·Lab on a Chip·M SolsonaM Odijk
Oct 25, 2017·Advanced Healthcare Materials·Shantanu PradhanJohn H Slater
Jul 19, 2020·Optics Express·J A Sarabia-AlonsoR Ramos-García
Jun 7, 2019·Lab on a Chip·Iago PereiroGovind V Kaigala
Jun 19, 2019·Lab on a Chip·Robyn H PritchardSalman Samson Rogers

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