Pulses of Ca2+ coordinate actin assembly and exocytosis for stepwise cell extension

Proceedings of the National Academy of Sciences of the United States of America
Norio TakeshitaR Fischer

Abstract

Many eukaryotic cells grow by extending their cell periphery in pulses. The molecular mechanisms underlying this process are not yet fully understood. Here we present a comprehensive model of stepwise cell extension by using the unique tip growth system of filamentous fungi. Live-cell imaging analysis, including superresolution microscopy, revealed that the fungus Aspergillus nidulans extends the hyphal tip in an oscillatory manner. The amount of F-actin and secretory vesicles (SV) accumulating at the hyphal tip oscillated with a positive temporal correlation, whereas vesicle amounts were negatively correlated to the growth rate. The intracellular Ca2+ level also pulsed with a positive temporal correlation to the amount of F-actin and SV at the hyphal tip. Two Ca2+ channels, MidA and CchA, were needed for proper tip growth and the oscillations of actin polymerization, exocytosis, and the growth rate. The data indicate a model in which transient Ca2+ pluses cause depolymerization of F-actin at the cortex and promote SV fusion with the plasma membrane, thereby extending the cell tip. Over time, Ca2+ diffuses away and F-actin and SV accumulate again at the hyphal tip. Our data provide evidence that temporally controlled actin poly...Continue Reading

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Citations

Mar 7, 2018·Proceedings of the National Academy of Sciences of the United States of America·Carlisle S BascomMagdalena Bezanilla
Nov 1, 2018·Nature Reviews. Microbiology·Zhenzhong Yu, Reinhard Fischer
Feb 28, 2019·International Microbiology : the Official Journal of the Spanish Society for Microbiology·Oier Etxebeste, Eduardo A Espeso
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Mar 22, 2019·Cancers·Karl KunzelmannRainer Schreiber
Apr 20, 2019·Frontiers in Microbiology·Monika S Fischer, N Louise Glass
Jun 11, 2020·Fungal biology and biotechnology·Mizuki YasuiNorio Takeshita
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Jul 23, 2020·Bioscience, Biotechnology, and Biochemistry·Shinobu TakagiShinichi Ohashi
Apr 13, 2018·Microbiology and Molecular Biology Reviews : MMBR·Meritxell RiquelmeReinhard Fischer
Sep 24, 2020·Life Science Alliance·Gayan AbeysingheNorio Takeshita
Dec 13, 2017·Fungal Genetics and Biology : FG & B·Norio Takeshita
Feb 20, 2021·Fungal Genetics and Biology : FG & B·Hye-Seon KimSeogchan Kang
Nov 2, 2019·Fungal Genetics and Biology : FG & B·Martin SchusterGero Steinberg
Mar 18, 2021·MBio·Sayumi FukudaNorio Takeshita
Apr 25, 2021·Science Advances·Runchen ZhaoKonstantinos Konstantopoulos
Jun 16, 2021·MBio·Valentin WernetReinhard Fischer
Feb 1, 2018·ACS Synthetic Biology·Taiji OkanoHiroaki Suzuki
Sep 8, 2021·The New Phytologist·Jacques Dumais

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