PUM2 Promotes Glioblastoma Cell Proliferation and Migration via Repressing BTG1 Expression

Cell Structure and Function
Yuanyu WangBaohua Jiao

Abstract

PUM2, an RNA binding protein, is known to promote stem cell proliferation via repressing expressions of cell cycle genes. Similar with stem cells, malignant cells are characterized by unlimited proliferation and remote migration. However, roles of PUM2 in cancer development are controversial. Here, we investigated PUM2's role in glioblastoma development and its relationship with the cell cycle regulator BTG1. Immunoblotting and RT-qPCR were used to evaluate protein expression level and transcript level, respectively. ShRNAs were designed to knock down PUM2 and BTG1 expression. CCK-8 assay was used to evaluate cell viability. Cell migration assay and evasion assay were used to evaluate metastatic capability of glioblastoma cell. RNA pull-down assay and RNA immunoprecipitation assay were used to test the interaction between PUM2 and BTG1 3'UTR. PUM2 expression is elevated in glioblastoma tumor tissues as well as glioblastoma cell lines. PUM2 knockdown remarkably suppresses glioblastoma cell proliferation and migration. In addition, PUM2 knockdown increases BTG1 expression. RNA pull-down assay and RNA immunoprecipitation assay show PUM2 binds to BTG1 3'UTR directly. Furthermore, knockdown of BTG1 reverses the effect of PUM2 knockd...Continue Reading

References

Nov 7, 2013·JAMA : the Journal of the American Medical Association·Antonio Omuro, Lisa M DeAngelis
May 8, 2018·Neuro-oncology·Aida KarachiMaryam Rahman
May 14, 2018·Journal of Neuro-oncology·Steinbjørn HansenKim Oren Gradel
Dec 24, 2018·Physical Review Letters·B P AbbottS Shandera
Jan 5, 2019·Physical Review Letters·D AdeyUNKNOWN Daya Bay Collaboration

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Citations

Jan 10, 2020·Recent Patents on Anti-cancer Drug Discovery·Junhe Zhang, Weihua Dong
Oct 13, 2020·Cancer Cell International·Yibing LiXiaoxin Ma
Aug 28, 2021·International Journal of Molecular Sciences·Rico SchieweckMichael A Kiebler

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