Pump and channel K (Rb+) fluxes in apoptosis of human lymphoid cell line U937

Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology
Alexey VereninovValentina Yurinskaya

Abstract

Ouabain-sensitive (OS) and -resistant (OR) Rb(+) influx was examined in three sublines of U937 cells to compare alterations of K(+) channel permeability and the Na(+),K(+)-ATPase pump leading to the shift in ion and water balance during apoptosis induced by 0.2 and 1microM staurosporine (STS) for 4-5 h. Cell K(+), Rb(+), Na(+) and Cl(-) content was determined by flame photometry and (36)Cl distribution. Changes in cell water content were monitored by measurement of buoyant cell density and distribution of [(3)H]-glycerol or 3-O-methyl-D-[(3)H]glucose. Apoptosis was detected by DNA flow cytometry and light microscopy of the native cells stained with acridine orange. Treatment with 0.2 microM STS for 5 hours led to mild apoptosis with 10-13 % cell dehydration and either moderate increase of channel mediated Rb(+) influx without significant changes in the pump activity or moderate decrease of pump Rb(+) influx without significant change of channel influx, depending on the cell line used. Treatment with 1 microM STS was followed by 18-23 % cell dehydration, a decrease of the pump activity and a small or insignificant increase in the OR Rb(+) influx in all studied sublines. It is concluded that moderate apoptotic cell shrinkage may ...Continue Reading

Citations

Apr 11, 2009·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Syed M QadriFlorian Lang
Dec 12, 2012·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Valentina YurinskayaAlexey Vereninov
Jun 21, 2011·Journal of Cellular Biochemistry·Syeda Tasneem TowhidFlorian Lang
Aug 2, 2017·Apoptosis : an International Journal on Programmed Cell Death·Valentina YurinskayaAlexey Vereninov
Nov 27, 2020·Frontiers in Cell and Developmental Biology·Valentina YurinskayaAlexey Vereninov

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis