Pure atmospheric pressure promotes an expression of osteopontin in human aortic smooth muscle cells

Biochemical and Biophysical Research Communications
K IizukaH Kawaguchi

Abstract

Although the types of pathophysiological stimulation that initiate an overexpression of OPN have yet to be determined, we hypothesized that mechanical stress is one of the candidates which initiates OPN expression in vascular smooth muscle cells. Cell proliferation assay indicated that a pure atmospheric pressure of 160 mmHg activated cell proliferation by 11% in human aortic smooth muscle cells (HASMC) compared to nonpressurized controls. Immunoblot analysis probed with an anti-OPN antibody demonstrated a 50% increase in OPN. Dual-luciferase reporter assay demonstrated that OPN promoter, corresponding to the -771 through -1 region of OPN gene, was highly responsive to pure atmospheric pressure by ten times that of the control. From these observations, we concluded that pure atmospheric pressure directly promotes an expression of OPN in HASMC, with these results also suggesting that high blood pressure-mediated mechanical compression is involved in the process of atherosclerosis and remodeling via OPN expression in HASMC.

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Citations

Jan 6, 2005·Journal of Zhejiang University. Science. B·Lei SunTakeshi Karino
Jan 17, 2004·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Kenji IizukaHideaki Kawaguchi
Apr 15, 2008·Journal of the American Society of Echocardiography : Official Publication of the American Society of Echocardiography·Lisa de las FuentesVíctor G Dávila-Román
Apr 7, 2010·Laboratory Investigation; a Journal of Technical Methods and Pathology·Peter FickertMichael Trauner
Aug 5, 2004·American Journal of Physiology. Endocrinology and Metabolism·Noriteru MoritaHideaki Kawaguchi
Feb 21, 2008·Acta Physiologica·M UenoyamaS Suzuki
Jan 7, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Janeta NikolovskiDavid J Mooney
May 11, 2004·World Journal of Gastroenterology : WJG·Zhong-Sheng ZhuShao-Liang Chen
Mar 10, 2017·Scientific Reports·Utako YokoyamaYoshihiro Ishikawa
Feb 2, 2021·Epidemiology and Infection·R K Sanayaima SinghR K Brojen Singh

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