PMID: 9546675Apr 18, 1998Paper

Purification and amino acid sequence of halystase from snake venom of Agkistrodon halys blomhoffii, a serine protease that cleaves specifically fibrinogen and kininogen

European Journal of Biochemistry
T MatsuiK Titani

Abstract

We have isolated a serine protease, halystase, from Agkistrodon halys blomhoffii venom by chromatography on DEAE-Sepharose, heparin-Sepharose and Q-Sepharose columns, and have determined the complete amino acid sequence by Edman degradation and by mass spectral analysis of peptides generated by enzymatic and chemical cleavage. The 238-residue sequence of halystase, containing N-linked carbohydrates (about 13%) at two sites showed significant similarity to other thrombin-like snake venom serine proteases (66-72%), mammalian tissue kallikrein (42%) and thrombin (26%). Halystase contained the tentative catalytic triad of His43, Asp88 and Ser184 common to all serine proteases and Asp178 in the primary substrate-binding site. Although halystase contained an RGD sequence at residues 181-183, it did not inhibit platelet aggregation induced by ADP or collagen. It hydrolyzed most efficiently a tissue-kallikrein substrate, prolylphenylalanylarginyl-4-methyl-coumaryl-7-amide, and released bradykinin from bovine kininogen. Halystase did not coagulate human plasma, but it cleaved the fibrinogen B beta chain at the carboxyl side of Arg42 and cleaved slowly the fibrogen A alpha chain. Fibrinogen thus treated gradually became insensitive to th...Continue Reading

Citations

Oct 12, 2000·Toxicon : Official Journal of the International Society on Toxinology·S Y ChoY S Kim
Oct 12, 2000·Toxicon : Official Journal of the International Society on Toxinology·Y S KohD S Kim
Sep 11, 2002·Toxicon : Official Journal of the International Society on Toxinology·Raquel K BortoletoRaghuvir K Arni
Mar 10, 2000·Biochimica Et Biophysica Acta·T MatsuiK Titani
Sep 28, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·E SiigurJ Siigur
Oct 12, 2010·Journal of Biomedicine & Biotechnology·S S HassonA A Al-Jabri
Apr 6, 2007·Toxicon : Official Journal of the International Society on Toxinology·Yang JinYun Zhang
Jun 1, 2005·Toxicon : Official Journal of the International Society on Toxinology·Solange M T Serrano, Rachid C Maroun
Oct 22, 2013·Toxicon : Official Journal of the International Society on Toxinology·Liliane Maria Fernandes de OliveiraRaghuvir K Arni
Mar 7, 2013·Toxicon : Official Journal of the International Society on Toxinology·Ying ZhengWen-Hui Lee
Jul 19, 2011·Toxicology and Applied Pharmacology·Yuling QiuByung Rae Jin
Dec 20, 2018·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Ângela Alice Amadeu MegaleWilmar Dias da Silva
Aug 27, 1999·Biochemical and Biophysical Research Communications·E SiigurJ Siigur

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