Purification and amino acid sequencing of NAF, a novel neutrophil-activating factor produced by monocytes

Biochemical and Biophysical Research Communications
A WalzM Baggiolini

Abstract

Human blood mononuclear cells were cultured for 24 h in the presence of LPS (100 ng per 5 x 10(6) cells), and a monocyte-derived neutrophil-activating factor (NAF) was purified to apparent homogeneity from the conditioned media. The purification consisted of ammonium sulphate precipitation, gel filtration, chromatography on phosphocellulose followed by hydroxylapatite, and reversed-phase HPLC on C4 and CN-propyl columns. Amino acid sequence analysis (32 of 50 presumed residues) shows that NAF is a novel peptide with little homology to known ones. Crude and pure NAF stimulated human neutrophils to release granule enzymes and to produce superoxide and H2O2.

References

Jun 1, 1976·Scandinavian Journal of Immunology·A Bøyum
Apr 1, 1985·The Journal of Clinical Investigation·J E PenningtonT H Lee
Aug 1, 1987·Biochemical Pharmacology·B Dewald, M Baggiolini
Mar 1, 1970·Journal of Molecular Biology·S B Needleman, C D Wunsch
Jan 1, 1984·Journal of Molecular Evolution·D F FengR F Doolittle
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C W CastorD A Walz
Sep 1, 1980·The Journal of Clinical Investigation·G W HunninghakeR G Crystal
Feb 1, 1980·The Journal of Clinical Investigation·W W MerrillH Y Reynolds

❮ Previous
Next ❯

Citations

Mar 26, 1999·European Journal of Immunology·W SchorrH U Zeilhofer
Oct 1, 1992·Proteins·J A StuckeyB F Edwards
Apr 1, 1993·Journal of Neuro-oncology·M TadaN de Tribolet
Jan 1, 1994·Biotherapy·B J Kullberg, J W van 't Wout
Jul 5, 1993·Experientia·H BangU Feige
Aug 1, 1996·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·E J MillerA B Cohen
Jan 1, 1995·Immunologic Research·L F Santamaria BabiK Blaser
Jan 1, 1990·Molecular Aspects of Medicine·A Shock, G J Laurent
May 1, 1989·Immunology Today·J WestwickR D Camp
Jan 1, 1990·International Journal of Immunopharmacology·P Stevens, D E Piazza
Mar 1, 1995·Clinics in Dermatology·J M Schröder
May 18, 2005·Burns : Journal of the International Society for Burn Injuries·Hong ZhengQing-Lian Xu
Jun 14, 2005·Molecular Immunology·Puttharat BaoprasertkulZhanjiang Liu
May 3, 2003·Biochemical and Biophysical Research Communications·Ludger StändkerSilke Mark
Jan 5, 2002·Atherosclerosis·William S ShinStanley G Rockson
Mar 22, 2002·Developmental and Comparative Immunology·Kerry J LaingChristopher J Secombes
Jan 1, 1996·Pharmacology & Therapeutics·F M Williams
Apr 13, 2000·Veterinary Immunology and Immunopathology·C L Galligan, B L Coomber
Apr 12, 2002·Veterinary Immunology and Immunopathology·Mhan Pyo YangEui Bae Jeung
May 6, 1998·Burns : Journal of the International Society for Burn Injuries·F L YehR H Fang
Jul 1, 1997·Journal of the American College of Cardiology·N MarxA Schömig
Jun 17, 2000·Clinics in Dermatology·J M Schröder
Jun 18, 2003·Trends in Immunology·Mark O HuisingB M Lidy Verburg-van Kemenade
Jun 24, 2003·Biochimica Et Biophysica Acta·Kensaku OhashiEmiko Sano
Mar 26, 2002·Nature Reviews. Immunology·Amanda E I Proudfoot

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.