Purification and Analytical Application of Vigna mungo Chitinase for Determination of Total Fungal Load of Stored Cereals

Applied Biochemistry and Biotechnology
PreetyVinita Hooda

Abstract

A novel chitinase from urd bean (Vigna mungo) seeds was purified up to homogeneity and optimized with respect to its optimum working conditions of pH, temperature, and substrate concentration. Overall, 145-fold purification with 70% yield of the purified chitinase was achieved. The notable features of the purified enzyme were its appreciative substrate affinity as well as catalytic efficiency, high thermo stability (70% retention of initial activity at 70 °C after 60 min of continuous exposure), and pretty good storage stability (half-life of 45 days at 5 °C). The enzyme was used for determination of total chitin contents of the stored cereals that in the absence of any insect infestation, were considered to be directly proportional to the total fungal load of the tested samples. The method was linear up to 7.0 mM with 0.04 mM as the limit of detection. Percent recoveries of added chitin were < 90.0% and within-day and between-day coefficients of variations were > 3.0% for all the samples. Chitin values in stored cereal samples obtained by the present method and the popular DNS method showed good correlation, the value for coefficient of determination (R2) being < 0.98. Overall, the method yielded acceptable sensitivity, reprod...Continue Reading

References

Apr 1, 1993·Bioscience, Biotechnology, and Biochemistry·T Yamagami, G Funatsu
Apr 1, 1995·Bioscience, Biotechnology, and Biochemistry·M OhtaG Funatsu
Jan 25, 2005·Bioscience, Biotechnology, and Biochemistry·Toki TairaMasanobu Ishihara
Mar 16, 2005·Protein Expression and Purification·Shaoyun WangXiuyun Ye
Jan 1, 1971·Plant Physiology·F B AbelesW H Habig
Feb 27, 2008·Journal of Agricultural and Food Chemistry·Devavratha H Rao, Lalitha R Gowda
Sep 11, 2008·Journal of Food Science·S Y WangP F Rao
Oct 24, 2008·Phytopathology·Kenneth G MooreGary A Payne
Feb 5, 2009·Applied Biochemistry and Biotechnology·Sanya Kudan, Rath Pichyangkura
Jun 24, 2009·Applied Microbiology and Biotechnology·Shaoyun WangPingfan Rao
Jul 14, 2009·Applied Biochemistry and Biotechnology·Marcelo Fernando KernGiancarlo Pasquali
Sep 21, 2013·Applied Biochemistry and Biotechnology· Preety, Vinita Hooda
Sep 1, 2015·Protein Expression and Purification·Delia SpanòRosaria Medda

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.