Purification and antitumour activity of a lipopeptide biosurfactant produced by Bacillus natto TK-1

Biotechnology and Applied Biochemistry
Xiao-Hong CaoGuo-Wei Huang

Abstract

An antitumour lipopeptide biosurfactant purified from Bacillus natto TK-1 was able to inhibit the proliferation of MCF-7 human breast-cancer cells in a dose- and time-dependent manner. The activity of lactate dehydrogenase release showed no significant difference between MCF-7 cells treated with lipopeptide and untreated controls. The antitumour activity of the lipopeptide in MCF-7 cells was associated with cell apoptosis determined by typical morphological changes and sub-G(1) peak in cell growth-phase distribution. The cell cycle was arrested at G(2)/M phase. In addition, the caspase activity assay revealed that lipopeptide-induced apoptosis in MCF-7 cells was associated with caspase 3.

Citations

Aug 26, 2014·Applied Biochemistry and Biotechnology·Arun Kumar PradhanBarada Kanta Mishra
Oct 17, 2014·Indian Journal of Microbiology·Alwar Ramanujam Padmavathi, Shunmugiah Karutha Pandian
Jan 20, 2016·The Journal of General and Applied Microbiology·Palaniyandi VelusamyJae-Seong So
Sep 23, 2014·Drug Discovery Today·Goutam DeyMahitosh Mandal
Nov 9, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Naxin SunWentan Tian
Nov 24, 2020·Critical Reviews in Food Science and Nutrition·Libo LiuBingcan Chen
Aug 10, 2019·International Journal of Biological Macromolecules·Balasubramani GovindasamyPerumal Pachiappan

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