PMID: 8947499Nov 15, 1996Paper

Purification and biochemical characterization of a vacuolar serine endopeptidase induced by glucose starvation in maize roots

The Biochemical Journal
F JamesP Raymond

Abstract

An endopeptidase (designated RSIP, for root-starvation-induced protease) was purified to homogeneity from glucose-starved maize roots. The molecular mass of the enzyme was 59 kDa by SDS/PAGE under reducing conditions and 62 kDa by gel filtration on a Sephacryl S-200 column. The isoelectric point of RSIP was 4.55. The purified enzyme was stable, with no auto-proteolytic activity. The enzyme activity was strongly inhibited by proteinaceous trypsin inhibitors, di-isopropylfluorophosphate, 3,4-dichloroisocoumarin and PMSF, suggesting that the enzyme is a serine protease. The maximum proteolytic activity against different protein substrates occurred at pH 6.5. With the exception of succinyl-Leu-Leu-Val-Tyr-4-methylcoumarin, no hydrolysis was detected with synthetic tryptic, chymotryptic or peptidylglutamate substrates. The determination of the cleavage sites in the oxidized B-Chain of insulin showed specificity for hydrophobic residues at the P2 and P3 positions, indicating that RSIP is distinct from other previously characterized maize endopeptidases. Both subcellular fractionation and immuno-detection in situ indicated that RSIP is localized in the vacuole of the root cells. RSIP is the first vacuolar serine endopeptidase to be id...Continue Reading

Citations

Dec 16, 2005·Biology of the Cell·Tatiana Lundgren RoseFrancis Marty
Jan 16, 2007·The Plant Journal : for Cell and Molecular Biology·Daniel OsunaMark Stitt
Jun 20, 2008·Phytochemistry·Vijay Kumar SinghMedicherla V Jagannadham
Jan 31, 2004·Cell Motility and the Cytoskeleton·Zhengyuan Wang, Thomas C Pesacreta
Oct 10, 2009·Plant Signaling & Behavior·Mariana RosaFernando E Prado
Sep 5, 2002·Physiologia Plantarum·Barry ThorntonAllan Sim
Dec 8, 2020·Frontiers in Plant Science·Kerui HuangXiaocheng Jiang
Mar 19, 1999·Biochemical and Biophysical Research Communications·S NiamkéB Colas
Feb 15, 2003·Plant Physiology·Sandra CortèsRenaud M Brouquisse
Jul 2, 2003·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Olivier GuionieBernard Colas
Jul 12, 2005·Plant Physiology and Biochemistry : PPB·Cecília M Antão, F Xavier Malcata
Jun 19, 2007·Journal of Agricultural and Food Chemistry·Ashok Kumar PatelMedicherla V Jagannadham
Dec 21, 2006·Journal of Agricultural and Food Chemistry·Monu PandeMedicherla V Jagannadham

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