Purification and biochemical properties of beta-lactamase produced by Proteus rettgeri.

Antimicrobial Agents and Chemotherapy
M MatsuuraS Mitsuhashi

Abstract

beta-Lactamase produced by Proteus rettgeri was found to be a typical cephalosporin beta-lactamase on the basis of its substrate hydrolysis profile. The enzyme activity was enhanced by prior treatment with an inducer. The enzyme was purified 166-fold by carboxymethyl-Sephadex column chromatography which indicated that its molecular weight was 42,000 +/- 2,000 and its isoelectric point was 8.7. Cefoperazone, cefoxitin, cefusulodin, cefmetazole, cefotaxime, 6059-S, FK749, YM-09330, carbenicillin, and cloxacillin were stable to this enzyme and possessed the function of competitive inhibition, as shown by their affinity for the beta-lactamase. The enzyme activity was inhibited by iodine, p-chloromerburibenzoate, and HG2+ ion. Clavulanic acid and CP-45899 displayed poor inhibitory activity toward this enzyme. The optimal pH was 8.0, and the optimal temperature was 50 degrees C.

References

Sep 1, 1977·Journal of Bacteriology·Y Fujii-KuriyamaS Sugawara
Aug 1, 1979·Antimicrobial Agents and Chemotherapy·H C NeuP Aswapokee
Dec 1, 1979·Antimicrobial Agents and Chemotherapy·N MatsubaraS Mitsuhashi
Feb 1, 1978·Antimicrobial Agents and Chemotherapy·K TsuchiyaH Nagatomo
Oct 1, 1978·Antimicrobial Agents and Chemotherapy·K TsuchiyaT Nishi
Jun 1, 1976·The Journal of Antimicrobial Chemotherapy·R B Sykes, M Matthew
May 1, 1977·Antimicrobial Agents and Chemotherapy·C Reading, M Cole
Mar 1, 1976·Antimicrobial Agents and Chemotherapy·C H O'CallaghanJ E Thornton
Jun 1, 1974·The Biochemical Journal·S G Waley
Jan 1, 1973·Advances in Microbial Physiology·M H Richmond, R B Sykes
Mar 1, 1980·Antimicrobial Agents and Chemotherapy·K HiraiS Mitsuhashi
Aug 1, 1974·Antimicrobial Agents and Chemotherapy·H C Neu

❮ Previous
Next ❯

Citations

Jan 1, 1985·Biotechnology & Genetic Engineering Reviews·A Coulson
Aug 1, 1981·Antimicrobial Agents and Chemotherapy·K HiraiS Mitsuhashi
Aug 1, 1982·Antimicrobial Agents and Chemotherapy·K KatsuS Mitsuhashi
Jul 1, 1987·Antimicrobial Agents and Chemotherapy·Y UtsuiS Mitsuhashi
Sep 1, 1988·Antimicrobial Agents and Chemotherapy·A TamuraS Mitsuhashi
Mar 1, 1989·Antimicrobial Agents and Chemotherapy·K Bush
Jun 1, 1995·Antimicrobial Agents and Chemotherapy·K BushA A Medeiros
Jan 13, 2009·Clinical Microbiology Reviews·George A Jacoby
Jan 1, 1982·Microbiology and Immunology·M TajimaS Sugawara

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

CRISPR Screens in Drug Resistance

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of CRISPR-Cas system in high-throughput genome-wide screens to identify genes that may confer drug resistance.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Beta-lactamase Inhibitors

Beta-lactamase inhibitors are a class of antibiotics that inhibit beta-lactamases, a family of enzymes involved in bacterial resistance to beta-lactam antibiotics. Here is the latest research.

Beta-lactamase Inhibitors (ASM)

Beta-lactamase inhibitors are a class of antibiotics that inhibit beta-lactamases, a family of enzymes involved in bacterial resistance to beta-lactam antibiotics. Here is the latest research.