Purification and biocompatibility of fermented hyaluronic acid for its applications to biomaterials

Biomaterials Research
Sung Chul ChoiChan-Wha Kim

Abstract

Hyaluronic acid (HA) is of importance due to its diverse applications in pharmaceuticals and medical devices such as dermal filler, adhesion barriers, carrier for cells and bioactive molecules as well as scaffold biomaterials for tissue engineering. Evaluations of purification and biocompatibility of HA are required for its applications to biomaterials. After synthesizing HA by fermentation of streptococcus zooepidemicus for 25 hr, extensively purification of the fermented broth was performed to remove impurities using a filtration process for insoluble components and cells, and diverse adsorbents for soluble impurities. Its in vitro biocompatibility has been evaluated by measurement of cell counting and assay of cell live and dead. 60% yield of white HA powder was obtained, having 15-17 dL/g intrinsic viscosity with a molecular weight of approximately 1,000 kDa. While low molecular weight impurities and insoluble impurities were successfully removed using a ultrafiltration membrane with 50 KDa molecular weight cut, endotoxins, high molecular weight proteins and nucleic acids were removed from the broth by employing adsorbents such as alumina and activated carbons. Alumina showed the best results for the removal of endotoxins, ...Continue Reading

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Citations

Jan 30, 2020·Nature Reviews. Microbiology·M Fata Moradali, Bernd H A Rehm
Nov 27, 2019·Biomaterials Research·Bo Min HongWon Ho Park
Feb 19, 2015·Journal of Biomedical Materials Research. Part a·Sung Chul ChoiChan-Wha Kim
Jun 14, 2020·Carbohydrate Polymers·Maha M AbdallahMaria do Rosário Bronze
Mar 17, 2021·Biomaterials·Alexander E G BakerMolly S Shoichet
Mar 20, 2021·International Journal of Biological Macromolecules·Bo Min HongWon Ho Park
Jun 14, 2021·International Journal of Biological Macromolecules·Bo Min HongWon Ho Park
Aug 10, 2021·Frontiers in Bioengineering and Biotechnology·Mario A Torres-AcostaCuauhtemoc Licona-Cassani

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Methods Mentioned

BETA
broth filtration
Light Scattering

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