PMID: 7011809Feb 1, 1981Paper

Purification and characterisation of a membrane-bound substance-P-degrading enzyme from human brain

European Journal of Biochemistry
C M LeeL L Iversen

Abstract

A membrane-bound enzyme which degrades substance P (an undecapeptide) has been purified from human brain. The properties of this enzyme suggest that it may be involved in the physiological inactivation of the peptide by neural tissues. Enzyme activity was extracted from a membrane fraction of human diencephalon with a non-ionic detergent, Brij 35, and activity was monitored by measuring the disappearance of added substance P using radioimmunoassay, bioassay or radiochemical assay. The enzyme was purified about 1000-fold by chromatography on DEAE-cellulose, hydroxyapatite and Sephadex gel filtration columns. To identify the cleavage sites in substance P, the peptide was incubated with the purified enzyme and the breakdown products were separated by reverse-phase high-performance liquid chromatography and identified by amino acid analysis. The results suggested that the enzyme preparation was functionally homogeneous and it cleaved substance P between Gln6-Phe7, Phe7-Phe8 and Phe8-Gly9, with no exopeptidase action. The enzyme had a pH optimum in the range 7--9 and was strongly inhibited by metal-chelating agents, but not affected by most other peptidase inhibitors; it can thus be classified as a neutral metallo-endopeptidase. The...Continue Reading

References

Nov 4, 1976·Nature·L L IversenI Kanazawa
Dec 23, 1976·Nature·C SchenkerS E Leeman
Jan 1, 1979·Biochemical Pharmacology·C M LeeL L Iversen
Nov 1, 1979·Biochemical Pharmacology·H BergerH Niedrich
Oct 26, 1979·European Journal of Pharmacology·A ArreguiL L Iversen
Aug 1, 1979·Journal of Neurochemistry·M OrlowskiS Wilk
Sep 12, 1979·Biochemical and Biophysical Research Communications·S Blumberg, V I Teichberg
Oct 1, 1977·Journal of Neurochemistry·A C CuelloL L Iversen
Dec 1, 1977·Naunyn-Schmiedeberg's Archives of Pharmacology·F LembeckG Schindler
Oct 1, 1978·Naunyn-Schmiedeberg's Archives of Pharmacology·F LembeckR Gamse
Feb 1, 1979·Journal of Neurochemistry·T N AkopyanA A Galoyan
Nov 1, 1976·The Journal of Cell Biology·Y J Wang, H R Mahler
Jul 8, 1975·Biochemical and Biophysical Research Communications·M Benuck, N Marks
Oct 1, 1972·Analytical Biochemistry·A H Wardi, G A Michos
Oct 1, 1972·Journal of Neurochemistry·H Y Yang, N H Neff
Apr 20, 1967·Biochemical and Biophysical Research Communications·I Schechter, A Berger
Aug 21, 1980·Nature·M R HanleyR Wade
Jan 1, 1980·Annual Review of Neuroscience·R A NicollS E Leeman
Jan 1, 1980·Journal of Neurochemistry·R F GilbertJ Wass
Feb 1, 1981·European Journal of Biochemistry·B E SandbergL L Iversen
Mar 1, 1980·Journal of Neurochemistry·T KatoT Nagatsu
Feb 15, 1962·The Japanese Journal of Physiology·K KATAOKA

❮ Previous
Next ❯

Citations

Mar 1, 1984·Neurochemical Research·A Pope, R A Nixon
Aug 27, 1986·Brain Research·P J Elliott, S D Iversen
Oct 15, 1982·European Journal of Pharmacology·J W Growcott, A V Tarpey
Nov 19, 1985·European Journal of Pharmacology·Z Y FriedmanZ Selinger
Sep 24, 1985·European Journal of Pharmacology·P Le GrevesT Hökfelt
Feb 6, 1990·European Journal of Pharmacology·J M HallI K Morton
Aug 25, 1995·European Journal of Pharmacology·S KhanA T Michael-Titus
Dec 1, 1990·Pharmacology, Biochemistry, and Behavior·T SakuradaK Kisara
Dec 1, 1983·Regulatory Peptides·J M Conlon, L Sheehan
Feb 1, 1986·Neuroscience Research·T KatoA Shimizu
Sep 1, 1982·Peptides·J M StewartW A Krivoy
Jan 1, 1988·Neurochemistry International·G M LittlewoodA J Turner
Dec 19, 1988·Neuroscience Letters·T SakuradaL Terenius
Apr 1, 1988·Brain Research Bulletin·R A Cridland, J L Henry
Oct 1, 1993·Journal of Dermatological Science·M KusuharaY Sasai
Jul 1, 1996·The International Journal of Biochemistry & Cell Biology·A M Khawaja, D F Rogers

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.