PMID: 39624Jun 19, 1979

Purification and characterisation of alpha-L-fucosidase from human placenta. pH-dependent changes in molecular size

Biochimica Et Biophysica Acta
B M Turner

Abstract

alpha-L-Fucosidase has been purified 12 000 fold from human placenta. The enzyme is a glycoprotein containing, by weight: 0.9% galactose; 1.9% mannose, 1.9% N-acetylglucosamine and 1.9% N-acetylneuraminic acid. Polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate separated proteins with molecular weights ot 55 000, 51 400 and 25 000. Resolution of the two larger protein bands varied with the gel system and these proteins may differ only in carbohydrate content. Gel filtration of te purified enzyme failed to separate the three proteins. Treatments with the cross-linking reagent dimethyl suberimidate prior to electrophoresis, resulted in a diminution of the original protein bands and the formation of oligomers with molecular weights of 80 000, 100 000, 130 000, and 144 000. These results suggest that the heavy (55 000 and 51 400) and light (25 000) proteins are structurally associated. The molecular weight of the native enzyme, measured by gel filtration, was dependent on the pH of the eluting buffer. At pH 5.0 or 6.0 a catalytically active peak was observed, with a molecular weight of 305 000. At pH 7.5 this peak was completely absent and the enzyme eluted as an asymmetrical peak with an apparent molecul...Continue Reading

References

Jan 1, 1986·Journal of Inherited Metabolic Disease·D M Swallow, D A Hopkinson
Apr 14, 1989·Clinica Chimica Acta; International Journal of Clinical Chemistry·L RogerM J Foglietti
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·N N ChuangK S Lin
Jan 1, 1991·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·S W Johnson, J A Alhadeff
Jun 8, 2001·European Journal of Biochemistry·O J CorderoF J Rodríguez-Berrocal
Jun 1, 1991·Clinical Biochemistry·R A DiCioccio, B A Gordon
Mar 31, 2018·Archives of Insect Biochemistry and Physiology·Natalia N PerrellaAdriana R Lopes
May 23, 2009·FEMS Microbiology Letters·Mangala A NadkarniNicholas A Jacques

Citations

Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·G E Davies, G R Stark

Related Concepts

Galactose Measurement
Placenta Specimen
Glycoproteins
Aggregation
Placenta
Mannose
Galactose
Dimethyl Suberimidate
Gel Chromatography
Purification Aspects

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Synapse Loss as Therapeutic Target in MS

As we age, the number of synapses present in the human brain starts to decline, but in neurodegenerative diseases this occurs at an accelerated rate. In MS, it has been shown that there is a reduction in synaptic density, which presents a potential target for treatment. Here is the latest research on synapse loss as a therapeutic target in MS.

Artificial Intelligence in Cardiac Imaging

Artificial intelligence (ai) techniques are increasingly applied to cardiovascular (cv) medicine in cardiac imaging analysis. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

Social Learning

Social learning involves learning new behaviors through observation, imitation and modeling. Follow this feed to stay up to date on the latest research.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Single Cell Chromatin Profiling

Techniques like ATAC-seq and CUT&Tag have the potential to allow single cell profiling of chromatin accessibility, histones, and TFs. This will provide novel insight into cellular heterogeneity and cell states. Discover the latest research on single cell chromatin profiling here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells.